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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 10

gen. nov. sp. nov., a diatom symbiont isolated from the phycosphere of Open Access

Abstract

A diatom-associated bacterium, designated as strain F10, was isolated from a pure culture of the pennate diatom A3 and has since been used to characterize molecular mechanisms of symbiosis between phytoplankton and bacteria, including interactions using diatom-derived azelaic acid. Its origin from a hypersaline environment, combined with its capacity for quorum sensing, biofilm formation, and potential for dimethylsulfoniopropionate methylation/cleavage, suggest it is within the family . Initial phylogenetic analysis of the 16S rRNA gene sequence placed this isolate within the genus, but recent genomic and phylogenomic analyses show strain F10 is a separate lineage diverging from the genus . The genomic DNA G+C content is 60.0 mol%. The predominant respiratory quinone is Q-10. The major fatty acids are C ω7 and C. Strain F10 also contains C3-OH and the furan-containing fatty acid 10,13-epoxy-11-methyl-octadecadienoate (9-(3-methyl-5-pentylfuran-2-yl)nonanoic acid). The major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Based on genomic, phylogenomic, phenotypic and chemotaxonomic characterizations, strain F10 represents a novel genus and species with the proposed name, gen. nov. sp. nov. The type strain is F10 (=NCMA B37=NCIMB 15470=NRIC 2002).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): diatom–bacteria symbiosis , phycosphere and Roseobacter
Funding
This study was supported by the:
  • Research Institute Centers, New York University Abu Dhabi (Award ADHPG-CG009)
    • Principle Award Recipient: ShadyA. Amin
  • Gordon and Betty Moore Foundation (Award GBMF9335)
    • Principle Award Recipient: ShadyA. Amin
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-10-27
2025-04-27
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Phycobacter azelaicus gen. nov. sp. nov., a diatom symbiont isolated from the phycosphere of Asterionellopsis glacialis
Int J Syst Evol Microbiol 73, 006104 (2023); https://doi.org/10.1099/ijsem.0.006104
/content/journal/ijsem/10.1099/ijsem.0.006104
/content/journal/ijsem/10.1099/ijsem.0.006104
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