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Volume 73, Issue 10

gen. nov., sp. nov., isolated from a marine invertebrate from the South China Sea No Access

Abstract

A Gram-stain-negative, facultative anaerobic, non-flagellated and oval-shaped (0.77–0.98 µm wide and 0.74–1.21 µm long) bacterial strain, designated XY-301, was isolated from a marine invertebrate collected from the South China Sea. Strain XY-301 grew at 15–37 °C (optimum, 30–35 °C) and at pH 7.0–8.5 (optimum, pH 8.0). The strain was slightly halophilic and it only grew in the presence of 0.5–6.5 % (w/v) NaCl (optimum, 2.5–3.5 %). Its predominant fatty acid (>10 %) was C 7. The predominant polar lipids of XY-301 were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, six unidentified aminolipids, three unidentified phospholipids and two unknown polar lipids. The respiratory quinone was Q-10. The genome of XY-301 was 4 979 779 bp in size, with a DNA G+C content of 61.3 mol%. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between XY-301 and SSK3-2 were 73.3, 14.5 and 53.5 %, respectively. Based on the results of phylogenetic, phenotypic, chemotaxonomic and genomic analyses, strain XY-301 is considered to represent a novel species and a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is XY-301 (=KCTC 72212=MCCC 1K03614).

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Keyword(s): Dapeng Nature Reserve , marine invertebrate , new species , Pacificoceanicola onchidii , Roseobacteraceae , taxonomy and whole genome sequence
Funding
This study was supported by the:
  • Natural Science Foundation of Guangdong Province (Award 2020A1515011117)
    • Principle Award Recipient: NotApplicable
  • Shenzhen science and technology application demonstration project (Award KJYY20180201180253571)
    • Principle Award Recipient: NotApplicable
  • the 62st-class General Financial Grant from the China Postdoctoral Science Foundation (Award No. 2017M622754)
    • Principle Award Recipient: NotApplicable
  • Scientific and Technical Innovation Council of Shenzhen (Award 20220811101028001)
    • Principle Award Recipient: LingyunHao
  • China National Key Research and Development Project (Award No. 2018YFA0902500)
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
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Pacificoceanicola onchidii gen. nov., sp. nov., isolated from a marine invertebrate from the South China Sea
Int J Syst Evol Microbiol 73, 006103 (2023); https://doi.org/10.1099/ijsem.0.006103
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