1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

sp. nov., isolated from the stem of a mangrove plant in Hainan No Access

Abstract

A novel strain, designated as HNM0663, was isolated from the stem of a mangrove plant () collected from the coast of Chengmai city, Hainan Island, PR China. On the basis of the alignment of 16S rRNA gene sequences, strain HNM0663 was closely related to LCR6-01 (98.67 %), YIM 33098 (98.12 %) and CMU-AB204 (97.93 %). Genome-based comparisons showed that strain HNM0663 was distinguished from its closest related species with 80.3 % average nucleotide identity and 20.2 % digital DNA–DNA hybridization values. The main cellular fatty acids were iso-C, iso-C and anteiso-C. The main menaquinones were MK-9 (H), MK-9 (H) and MK-8 (H). The predominant phospholipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine. Based on these polyphasic taxonomy results, strain HNM0663 should represent a novel species, for which the name sp. nov. is proposed. The type strain is HNM 0663 (=CCTCC AA 2019075=LMG 31909).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Actinomycetota , Avicennia marina , mangrove plant and Streptomyces chengmaiensis sp. nov.
Funding
This study was supported by the:
  • Degree and Graduate Education Reform Project of Innovation of Project of Guangxi Graduate Educaiton (Award JGY2022187)
    • Principle Award Recipient: ShuangqingZhou
  • Guangxi Natural Science Foundation of China (Award 2021GXNSFAA196044)
    • Principle Award Recipient: ShuangqingZhou
© 2023 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.006101
2023-10-27
2024-05-09
Loading full text...

Full text loading...

References

  1. Xu D-B, Ye W-W, Han Y, Deng Z-X, Hong K. Natural products from mangrove actinomycetes. Mar Drugs 2014; 12:2590–2613 [View Article] [PubMed]
     [Google Scholar]
  2. Waksman SA, Henrici AT. The nomenclature and classification of the Actinomycetes. J Bacteriol 1943; 46:337–341 [View Article] [PubMed]
     [Google Scholar]
  3. Law JWF, Tan L-H, Letchumanan V, Hong K-W, Ser H-L et al. Streptomyces griseiviridis sp. nov., a novel “modern actinobacteria” isolated from Malaysia mangrove soil. Prog Micobes Mol Biol 2023; 6:a0000270 [View Article]
     [Google Scholar]
  4. Zhu P, Xu Y, Fu J, Liao Y. Streptomyces qinzhouensis sp. nov., a mangrove soil actinobacterium. Int J Syst Evol Microbiol 2020; 70:1800–1804 [View Article] [PubMed]
     [Google Scholar]
  5. Law J-F, Ser H-L, Ab Mutalib N-S, Saokaew S, Duangjai A et al. Streptomyces monashensis sp. nov., a novel mangrove soil actinobacterium from East Malaysia with antioxidative potential. Sci Rep 2019; 28:30–56 [View Article] [PubMed]
     [Google Scholar]
  6. Ivantiskaya LP, Singal SM, Bibikova MV, Vostrov SN. Directed isolation of Micromonospora on selective media with gentamicin. Antibiotiki 1978; 23:690–692
     [Google Scholar]
  7. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 2012; 19:455–477 [View Article] [PubMed]
     [Google Scholar]
  8. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M et al. Introducing EzTaxon-E: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article] [PubMed]
     [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  10. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article] [PubMed]
     [Google Scholar]
  11. Rzhetsky A, Nei M. A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 1992
     [Google Scholar]
  12. Tamura K, Stecher G, Kumar S. Mega11: Molecular Evolutionary Genetics Analysis version 11. Mol Biol Evol 2021; 38:3022–3027 [View Article]
     [Google Scholar]
  13. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
     [Google Scholar]
  14. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
     [Google Scholar]
  15. Meier-Kolthoff JP, Carbasse JS, Peinado-Olarte RL, Göker M. TYGS and LPSN: a database tandem for fast and reliable genome-based classification and nomenclature of prokaryotes. Nucleic Acids Res 2022; 50:D801–D807 [View Article] [PubMed]
     [Google Scholar]
  16. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016; 32:929–931 [View Article] [PubMed]
     [Google Scholar]
  17. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article] [PubMed]
     [Google Scholar]
  18. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  19. Blin K, Shaw S, Augustijn HE, Reitz ZL, Biermann F et al. antiSMASH 7.0: new and improved predictions for detection, regulation, chemical structures and visualisation. Nucleic Acids Res 2023; 51:W46–W50 [View Article] [PubMed]
     [Google Scholar]
  20. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
     [Google Scholar]
  21. Kelly KL. Inter-Society Color Council - National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office; 1964
     [Google Scholar]
  22. Huang X, Kong F, Zhou S, Huang D, Zheng J et al. Streptomyces tirandamycinicus sp. nov., a novel marine sponge-derived actinobacterium with antibacterial potential against Streptococcus agalactiae. Front Microbiol 2019; 10:482 [View Article] [PubMed]
     [Google Scholar]
  23. Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH et al. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983; 129:1743–1813 [View Article] [PubMed]
     [Google Scholar]
  24. Lechevalier MP, Lechevalier HA. The Chemotaxonomy of Actinomycetes. In Dietz A, Thayer DW. eds Actinomycete Taxonomy (Special Publication No. 6) Arlington, VA: Society of Industrial Microbiology; 1980 pp 227–291
     [Google Scholar]
  25. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  26. Sasser M. Technical Note 101: Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  27. Saeng-In P, Phongsopitanun W, Savarajara A, Tanasupawat S. Streptomyces lichenis sp. nov., isolated from lichen. Int J Syst Evol Microbiol 2018; 68:3641–3646 [View Article] [PubMed]
     [Google Scholar]
  28. Lee ZY, Ng ZY, Mohd Nor MN, Teo WFA, Tan GYA. Streptomyces solincola sp. nov., isolated from soil in Malaysia. Int J Syst Evol Microbiol 2022; 72:005594 [View Article] [PubMed]
     [Google Scholar]
  29. JiangY LW, Li W, Xu P, Li M, Wen M et al. Streptomyces nanningensis sp. nov. a novel Streptomycete from forest soil. Antonie van Leeuwenhoek 2005; 87:215–220 [View Article] [PubMed]
     [Google Scholar]
  30. Sujarit K, Kudo T, Ohkuma M, Pathom-Aree W, Lumyong S. Streptomyces palmae sp. nov., isolated from oil palm (Elaeis guineensis) rhizosphere soil. Int J Syst Evol Microbiol 2016; 66:3983–3988 [View Article] [PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.006101
Loading
Streptomyces chengmaiensis sp. nov., isolated from the stem of a mangrove plant in Hainan
Int J Syst Evol Microbiol 73, 006101 (2023); https://doi.org/10.1099/ijsem.0.006101
/content/journal/ijsem/10.1099/ijsem.0.006101
/content/journal/ijsem/10.1099/ijsem.0.006101
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error