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Abstract

Strain CBA3108 is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108 are non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25–45 °C (optimum, 35 °C); pH 6–9 (optimum, pH 6); and in the presence of 0–6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C iso and C iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain CBA3108 forms a phyletic lineage distinct from other closely related species within the genus . It was found to be most closely related to ATCC 25577 (98.27 % 16S rRNA gene sequence similarity) and other strains within the genus (≤98.0 %). The genomic DNA G+C content of strain CBA3108 was 63.2 mol%. The DNA–DNA hybridization values of strain CBA3108 with ATCC 25577, WCA-380-WT-3A and subsp. DSM 1897 were 33.6, 21.7 and 22.7 %, respectively. Its phenotypic, chemotaxonomic and molecular properties support the hypothesis that strain CBA3108 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is CBA3108 (=KACC 22889=JCM 35966).

Funding
This study was supported by the:
  • Ministry of Science and ICT, South Korea (Award 2021R1C1C1013859)
    • Principle Award Recipient: SeHee Lee
  • World Institute of Kimchi (Award KE2301-1-1)
    • Principle Award Recipient: TaeWoong Whon
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2023-11-01
2024-12-14
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References

  1. Wylensek D, Hitch TCA, Riedel T, Afrizal A, Kumar N et al. A collection of bacterial isolates from the pig intestine reveals functional and taxonomic diversity. Nat Commun 2020; 11:6389 [View Article] [PubMed]
    [Google Scholar]
  2. Bhatia A, Maisonneuve J-F, Persing DH. Propionibacterium acnes and chronic diseases. In The Infectious Etiology of Chronic Diseases: Defining the Relationship Enhancing the Research, and Mitigating the Effects: Workshop Summary The National Academies Press; 2004
    [Google Scholar]
  3. Dekio I, Sakamoto M, Suzuki T, Yuki M, Kinoshita S et al. Cutibacterium modestum sp. nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense. Int J Syst Evol Microbiol 2020; 70:2457–2462 [View Article] [PubMed]
    [Google Scholar]
  4. Gilchrist T. A bacterial and microscopical study of over three hundred vesicular and pustular lesions of the skin with a research upon the etiology of acne. Johns Hopkins Hospital Reports 1900; 9:408–430
    [Google Scholar]
  5. Douglas HC, Gunter SE. The taxonomic position of Corynebacterium acnes. J Bacteriol 1946; 52:15–23 [View Article] [PubMed]
    [Google Scholar]
  6. Scholz CFP, Kilian M. The natural history of cutaneous propionibacteria, and reclassification of selected species within the genus Propionibacterium to the proposed novel genera Acidipropionibacterium gen. nov., Cutibacterium gen. nov. and Pseudopropionibacterium gen. nov. Int J Syst Evol Microbiol 2016; 66:4422–4432 [View Article] [PubMed]
    [Google Scholar]
  7. Levy PY, Fenollar F, Stein A, Borrione F, Cohen E et al. Propionibacterium acnes postoperative shoulder arthritis: an emerging clinical entity. Clin Infect Dis 2008; 46:1884–1886 [View Article] [PubMed]
    [Google Scholar]
  8. Ahle CM, Feidenhansl C, Brüggemann H. Cutibacterium acnes. Trends Microbiol 2023; 31:419–420 [View Article] [PubMed]
    [Google Scholar]
  9. Corvec S. Clinical and biological features of Cutibacterium (formerly Propionibacterium) avidum, an underrecognized microorganism. Clin Microbiol Rev 2018; 31:e00064-17 [View Article] [PubMed]
    [Google Scholar]
  10. Lane DJ. 16S/23S rRNA Sequencing New York: John Wiley & Sons; 1991
    [Google Scholar]
  11. Kim YB, Kim JY, Kim J, Song HS, Whon TW et al. Aminipila terrae sp. nov., a strictly anaerobic bacterium isolated from river sediment. Arch Microbiol 2021; 203:3163–3169 [View Article] [PubMed]
    [Google Scholar]
  12. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
    [Google Scholar]
  13. Thompson JD, Gibson TJ, Higgins DG. Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinformatics 2002; Chapter 2:Unit [View Article] [PubMed]
    [Google Scholar]
  14. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
    [Google Scholar]
  15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
    [Google Scholar]
  16. Koren S, Walenz BP, Berlin K, Miller JR, Bergman NH et al. Canu: scalable and accurate long-read assembly via adaptive K-MER weighting and repeat separation. Genome Res 2017; 27:722–736 [View Article] [PubMed]
    [Google Scholar]
  17. Walker BJ, Abeel T, Shea T, Priest M, Abouelliel A et al. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS One 2014; 9:e112963 [View Article] [PubMed]
    [Google Scholar]
  18. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article] [PubMed]
    [Google Scholar]
  19. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP et al. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res 2016; 44:6614–6624 [View Article] [PubMed]
    [Google Scholar]
  20. Kim J, Na SI, Kim D, Chun J. UBCG2: up-to-date bacterial core genes and pipeline for phylogenomic analysis. J Microbiol 2021; 59:609–615 [View Article] [PubMed]
    [Google Scholar]
  21. Price MN, Dehal PS, Arkin AP. FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol 2009; 26:1641–1650 [View Article] [PubMed]
    [Google Scholar]
  22. Lee I, Ouk Kim Y, Park S-C, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article] [PubMed]
    [Google Scholar]
  23. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
    [Google Scholar]
  24. Smibert R. Phenotypic characterization. In Methods for General and Molecular Bacteriology Wiley; 1994
    [Google Scholar]
  25. Choi EJ, Jin HM, Kim KH, Jeon CO. Salimicrobium jeotgali sp. nov., isolated from salted, fermented seafood. Int J Syst Evol Microbiol 2014; 64:3624–3630 [View Article] [PubMed]
    [Google Scholar]
  26. Gaby WL, Hadley C. Practical laboratory test for the identification of Pseudomonas aeruginosa. J Bacteriol 1957; 74:356–358 [View Article] [PubMed]
    [Google Scholar]
  27. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42:457–469 [View Article]
    [Google Scholar]
  28. Miller L, Berger T. Bacteria identification by gas chromatography of whole cell fatty acids. Hewlett-Packard Application Note 1985; 228:1–8
    [Google Scholar]
  29. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
  30. Greenman J, Holland KT, Cunliffe WJ. Effects of pH on biomass, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture. J Gen Microbiol 1983; 129:1301–1307 [View Article] [PubMed]
    [Google Scholar]
  31. Schlecht S, Freudenberg MA, Galanos C. Culture and biological activity of Propionibacterium acnes. Infection 1997; 25:247–249 [View Article] [PubMed]
    [Google Scholar]
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