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Volume 73, Issue 11

sp. nov., isolated from horse faeces No Access

Abstract

Strain CBA3108 is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108 are non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25–45 °C (optimum, 35 °C); pH 6–9 (optimum, pH 6); and in the presence of 0–6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C iso and C iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain CBA3108 forms a phyletic lineage distinct from other closely related species within the genus . It was found to be most closely related to ATCC 25577 (98.27 % 16S rRNA gene sequence similarity) and other strains within the genus (≤98.0 %). The genomic DNA G+C content of strain CBA3108 was 63.2 mol%. The DNA–DNA hybridization values of strain CBA3108 with ATCC 25577, WCA-380-WT-3A and subsp. DSM 1897 were 33.6, 21.7 and 22.7 %, respectively. Its phenotypic, chemotaxonomic and molecular properties support the hypothesis that strain CBA3108 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is CBA3108 (=KACC 22889=JCM 35966).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cutibacterium equinum , horse faeces , new taxon and taxonomy
Funding
This study was supported by the:
  • Ministry of Science and ICT, South Korea (Award 2021R1C1C1013859)
    • Principle Award Recipient: SeHee Lee
  • World Institute of Kimchi (Award KE2301-1-1)
    • Principle Award Recipient: TaeWoong Whon
© 2023 The Authors
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Cutibacterium equinum sp. nov., isolated from horse faeces
Int J Syst Evol Microbiol 73, 006099 (2023); https://doi.org/10.1099/ijsem.0.006099
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