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Volume 73, Issue 10

sp. nov., isolated from a bleached scleractinian coral No Access

Abstract

A Gram-stain-negative, aerobic, rod-shaped, nonmotile and yellow-pigmented bacterium designated E7-10 was isolated from a bleached scleractinian coral . Strain E7-10 grew with 1.0–8.0 % (w/v) NaCl (optimum, 4.0 %), at 18–41 °C (optimum, 28 °C) and at pH 6.0–10.0 (optimum, pH 8.0). Phylogenetic analysis using 16S rRNA gene sequences revealed that E7-10 formed a lineage within the genus , but it was distinct from the closest species ‘’ PM5-8, showing 98.01 % sequence similarity. The predominant cellular fatty acids of E7-10 were summed feature 8 (26.7 %), C 7 11-methyl (26.2 %), C (20.8 %) and C cyclo 8 (17.9 %). The major respiratory quinone was Q-10. The polar lipids mainly comprised phosphatidylethanolamine, two glycolipids and five phospholipids. The genome size of E7-10 was 5.58 Mb with G+C content 60.27 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between the genomes of strains E7-10 and PM5-8 were 19.50 and 75.95 %, respectively, which were both below the defined cutoff values (70 % and 95–96 %, respectively) for species delimitation. Thus, strain E7-10 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is E7-10 (=JCM 35852=MCCC 1K08229).

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  • Accepted:
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Keyword(s): Hoeflea poritis sp. nov. , novel species , Porites lutea and scleractinian coral
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 42267018)
    • Principle Award Recipient: QiaoyanWei
  • Natural Science Foundation of Shenzhen City (Award JCYJ20200109144803833)
    • Principle Award Recipient: LinCai
  • National Key Research and Development Program of China (Award 2019YFE0198500)
    • Principle Award Recipient: LinCai
© 2023 The Authors
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2023-10-20
2024-05-08
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Hoeflea poritis sp. nov., isolated from a bleached scleractinian coral Porites lutea
Int J Syst Evol Microbiol 73, 006097 (2023); https://doi.org/10.1099/ijsem.0.006097
/content/journal/ijsem/10.1099/ijsem.0.006097
/content/journal/ijsem/10.1099/ijsem.0.006097
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