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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

sp. nov., a marine bacterium isolated from the coast of Qingdao No Access

Abstract

A Gram-stain-negative, facultative anaerobic, rod-shaped strain, named SDRW27, was isolated from offshore seawater collected near Qingdao. Strain SDRW27 was able to grow at 16–37 °C (optimum, 28 °C), pH 6.0–9.0 (optimum, pH 6.0) and in the presence of 1–7 % (w/v) NaCl (optimum, 3 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SDRW27 was most closely related to H01100410B (97.89 % sequence similarity), H01100409B (97.89 %) and ATCC 25521 (97.82 %). The predominant fatty acids were summed feature 3 (C ω7 and/or iso-C 2-OH), summed feature 8 (C 7 and/or C 6) and C. The polar lipids of strain SDRW27 comprised phosphatidylglycerol, phosphatidylinositol dimannoside, phosphatidylcholine, phosphatidylethanolamine and three unidentified lipids. The major respiratory quinone was ubiquinone-8. The G+C content was 47.71 mol%. The genome size was 5.84 Mbp, including 85 contigs with an N50 value of 223 542. The average nucleotide identity (ANI) values of SDRW27 with its three most similar strains, H01100410B, H01100409B and ATCC 25521, were 71.36, 71.58 and 72.23 %, respectively (all lower than the 95–96 % ANI threshold), and the DNA–DNA hybridization (DDH) values were 20.4, 20.8 and 20.4 % (all lower than the 70 % DDH threshold). The obtained results of polyphasic analysis demonstrate that strain SDRW27 represents a novel species, for which the name sp. nov. is proposed. The type strain is SDRW27 (=MCCC 1K06286=KCTC 82892).

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Keyword(s): novel species , Photobacterium obscurum , polyphasic analysis , Qingdao offshore and seawater
Funding
This study was supported by the:
  • Postdoctoral Innovation Program of Shandong Province (Award SDCX-ZG-202202035)
    • Principle Award Recipient: XiaoleiWang
  • Qingdao Postdoctoral Scientific Research Foundation (Award QDBSH20220202165)
    • Principle Award Recipient: XiaoleiWang
  • Fundamental Research Funds for the Central Universities (Award 202172002)
    • Principle Award Recipient: Xiao-HuaZhang
  • National Natural Science Foundation of China (Award 92251303)
    • Principle Award Recipient: Xiao-HuaZhang
  • National Natural Science Foundation of China (Award 41730530)
    • Principle Award Recipient: Xiao-HuaZhang
  • National Key Research and Development Program of China (Award 2018YFE0124100)
    • Principle Award Recipient: Xiao-HuaZhang
© 2023 The Authors
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Photobacterium obscurum sp. nov., a marine bacterium isolated from the coast of Qingdao
Int J Syst Evol Microbiol 73, 006096 (2023); https://doi.org/10.1099/ijsem.0.006096
/content/journal/ijsem/10.1099/ijsem.0.006096
/content/journal/ijsem/10.1099/ijsem.0.006096
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