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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

sp. nov. and sp. nov., isolated from desert soil No Access

Abstract

Two novel bacterial strains, designated as SYSU D00344 and SYSU D00433, were isolated from soil of Gurbantunggut Desert in Xinjiang, north-west PR China. Cells of both strains were Gram-stain-negative, aerobic, short-rod-shaped, catalase-positive and non-motile. Oxidase activities of SYSU D00344 and SYSU D00433 were negative and positive, respectively. Optimal growth occurred at 30 °C, with 0–0.5 % (w/v) NaCl and at pH 7.0. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that they represented members of the genus and were closely related to NBS58-1. The results of phylogenomic analysis indicated that the two strains formed two independent and robust branches distinct from all reference type strains. The analyses of average nucleotide identity (ANI), digital DNA–DNA hybridisation (dDDH) values and 16S rRNA gene similarities between the two strains and their relatives further demonstrated that SYSU D00344 and SYSU D00433 represented two different novel genospecies. The polar lipids consisted of phosphatidylethanolamine, one unidentified glycolipid, two unidentified aminophospholipids, and two or four unidentified lipids. MK-7 was the only respiratory quinone. The major fatty acids (>10 %) for both strains were identified as iso-C, anteiso-C and summed feature 3 (Cω7 and/or Cω6), as well as summed feature 4 (anteiso-CB and/or iso-CI) for SYSU D00344 and Cω5 for SYSU D00433. On the basis of the phylogenetic, phenotypic, chemotaxonomic and genotypic characteristics, we propose sp. nov. and sp. nov. as two novel species in the genus . The type strains are SYSU D00344 (=CGMCC 1.8625=MCCC 1K04971=KCTC 82274) and SYSU D00433 (=CGMCC 1.8617=MCCC 1K04982=KCTC 82277), respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Gurbantunggut Desert , polyphasic taxonomy , Rufibacter aurantiacus , Rufibacter roseolus and whole-genome sequencing
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 32000005)
    • Principle Award Recipient: LeiDong
  • National Natural Science Foundation of China (Award 32061143043)
    • Principle Award Recipient: Wen-JunLi
  • Guangdong Basic and Applied Basic Research Foundation (Award 2023A1515012020)
    • Principle Award Recipient: ShuaiLi
  • the Third Xinjiang Scientific Expedition Program (Award 2022xjkk1200)
    • Principle Award Recipient: LeiDong
© 2023 Sun Yat-Sen University
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2023-10-20
2024-05-08
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Rufibacter roseolus sp. nov. and Rufibacter aurantiacus sp. nov., isolated from desert soil
Int J Syst Evol Microbiol 73, 006093 (2023); https://doi.org/10.1099/ijsem.0.006093
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