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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

gen. nov. sp. nov., a novel bacterium isolated from sediment in the Pearl River Estuary and reclassification of five and four species No Access

Abstract

An aerobic, Gram-negative, non-motile, yellow-to-orange pigmented and round bacterium, designated strain SCSIO 72103, was isolated from sediment collected in the Pearl River Estuary, Guangdong Province, PR China and subjected to a polyphasic taxonomic study. Growth occurred at 20–37 °C (optimum, 28 °C), pH 6–8 (optimum, pH 7) and with 1–5.5% NaCl (optimum, 1–3 %). Comparative 16S rRNA gene analysis indicated that strain SCSIO 72103 had the highest similarities to SNL9 (94.7 %) and SW105 (94.2 %). Phylogenetic analysis based 16S rRNA gene sequences showed that strain SCSIO 72103 formed a single clade with SW105. Strain SCSIO 72103 contained iso-C as the major fatty acid and the predominant respiratory quinone was menaquinone MK-6. These characteristics are consistent with those of SNL9 and SW105. Phosphatidylethanolamine, most notably, unidentified aminolipid and unidentified aminophospholipid were major polar lipids. Strain SCSIO 72103 had a single circular chromosome of 2.96 Mb with a DNA G+C content of 35.1 mol%. The average nucleotide identity, average amino acid identity (AAI) and digital DNA–DNA hybridization values showed that the pairwise similarities between SCSIO 72103 and the type strains of SNL9 and SW105 were 78.5–80.5 %, 79.0–81.4 % and 22.7–22.8 %, respectively. The AAI values between species in this clade and the type species of and were below the 65 % threshold, indicating that these species belong to a novel genus. On the basis of phylogenetic, physiological and chemotaxonomic characteristics, strain SCSIO 72103 represents a new species of a novel genus, for which the name gen. nov. sp. nov. is proposed. The type strain is SCSIO 72103 (=KCTC 92043=MCCC 1K06659). It is also proposed that nine known species in the genera and are reclassified as species.

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Keyword(s): Paenimyroides aestuarii sp. nov. , polyphasic taxonomy and sediment
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No. 32170018)
    • Principle Award Recipient: Xin-PengTian
  • Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (Award 2019BT02Y262)
    • Principle Award Recipient: Xin-PengTian
  • Natural Science Foundation of Hainan Province (Award 321CXTD447)
    • Principle Award Recipient: Xin-PengTian
© 2023 The Authors
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2023-10-26
2024-10-04
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Paenimyroides aestuarii gen. nov. sp. nov., a novel bacterium isolated from sediment in the Pearl River Estuary and reclassification of five Flavobacterium and four Myroides species
Int J Syst Evol Microbiol 73, 006091 (2023); https://doi.org/10.1099/ijsem.0.006091
/content/journal/ijsem/10.1099/ijsem.0.006091
/content/journal/ijsem/10.1099/ijsem.0.006091
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