1887

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Volume 73, Issue 10

sp. nov., a novel bacterium isolated from rhizosphere of (Bunge) Freitag & Schütze No Access

Abstract

Light yellowish-white colonies of a bacterial strain, designated LNNU 24178, were isolated from the rhizosphere soil of halophyte (Bunge) Freitag and Schütze grown at Shihezi district, Xinjiang, PR China. Cells were Gram-stain-negative, non-flagellum-forming, rod-shaped and non-motile. The results of phylogenetic analysis based on the 16S rRNA gene sequence indicated that LNNU 24178 represented a member of the genus and shared the highest sequence similarity with CGMCC 1.13927 (97.1 %) and lower sequence similarity (< 97.0 %) to other known species. The genomic DNA G+C content of LNNU 24178 was 68.8 %. The average nucleotide identity (ANI) values between LNNU 24178 and CGMCC 1.13927, DSM 12574, 26-35 and HB2 were 78.7, 78.6, 78.4 and 80.0 %, respectively. The digital DNA–DNA hybridisation (dDDH) values between LNNU 24178 and CGMCC 1.13927, DSM 12574, 26-35 and HB2 were 22.0, 22.3, 22.2 and 23.5 %, respectively. The respiratory quinone detected in LNNU 24178 was ubiquinone-8 (Q-8). The major fatty acids (> 5.0 %) of LNNU 24178 were identified as iso-C (33.9 %), iso-C (8.7 %), iso-C (6.2 %), iso-C (5.7 %), C (5.3 %) and summed feature 9 (iso-Cω9/10-methyl C) (21.1 %). The major polar lipids of LNNU 24178 were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), one unidentified phospholipid (PL), one unidentified glycolipid (GL) and three unidentified lipids. According to the data obtained from phenotypic, chemotaxonomic and phylogenetic analyses, strain LNNU 24178 represents a novel species of the genus , for which the name sp. nov. is proposed, with LNNU 24178 (= CGMCC 1.17331= KCTC 62251) as the type strain.

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Keyword(s): Luteimonas suaedaesp. nov , phylogenetic analysis and rhizosphere soil
Funding
This study was supported by the:
  • Educational Department of Liaoning Province (Award LJKMZ20221427)
    • Principle Award Recipient: HongfeiWang
  • Applied Basic Research Project of Liaoning Province (Award 2023JH2/101300167)
    • Principle Award Recipient: HongfeiWang
© 2023 Sun Yat-Sen University
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2023-10-13
2024-05-08
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Luteimonas suaedae sp. nov., a novel bacterium isolated from rhizosphere of Suaeda aralocaspica (Bunge) Freitag & Schütze
Int J Syst Evol Microbiol 73, 006088 (2023); https://doi.org/10.1099/ijsem.0.006088
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