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Volume 73, Issue 10

sp. nov., a slightly halophilic bacterium from a hypersaline lake No Access

Abstract

A novel halophilic bacterium, strain 71-i, was isolated from Inche-Broun hypersaline lake in Golestan province, in the north of Iran. It was a Gram-stain-negative, non-endospore forming, rod-shaped bacterium. It grew at 4–40 °C (optimum 30 °C), pH 6.0–11.0 (optimum pH 7.5) and with 0.5–15 % (w/v) NaCl [optimum 3 % (w/v) NaCl]. The results of phylogenetic analyses based on the 16S rRNA gene sequence comparison indicated its affiliation to the genus and the low percentage of identity with the most closely related species (97.5 %), indicated its placement as a novel species within this genus. Digital DNA–DNA hybridization (dDDH) values and average nucleotide identity (ANI) analyses of this strain against closely related species confirmed its condition of novel taxon. On the other hand, the percentage of the average amino acid identity (AAI) affiliated strain 71-i within the genus . The DNA G+C content of this isolate was 57.7 mol%. The major fatty acids were C and Cω and/or C ω6. Ubiquinone-9 was the major isoprenoid quinone and diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) were the main polar lipids of this strain. On the basis of the phylogenomic and phenotypic (including chemotaxonomic) features, we propose strain 71-i (= IBRC M 11023 = CECT 30160 = LMG 29252) as the type strain of a novel species within the genus , with the name sp. nov. Genomic detections of this strain in various metagenomic databases indicate that it is a relatively abundant species in environments with low salinities (approximately 5 % salinity), but not in hypersaline habitats with high salt concentrations.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): extremophiles , halophiles , hypersaline lake , Marinobacter , taxogenomics and taxonomy
Funding
This study was supported by the:
  • MCIN/AEI/10.13039/501100011033 (Award PID2020-118136GB-I00)
    • Principle Award Recipient: NotApplicable
  • Iranian National Science Foundation (INSF) (MAA).
    • Principle Award Recipient: NotApplicable
  • Research Council of the University of Tehran
    • Principle Award Recipient: NotApplicable
  • Consejería de Conocimiento, Investigación y Universidad, Junta de Andalucía (Award P20_01066 and BIO-213)
    • Principle Award Recipient: AntonioVentosa
© 2023 The Authors
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2023-10-27
2024-05-03
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Marinobacter iranensis sp. nov., a slightly halophilic bacterium from a hypersaline lake
Int J Syst Evol Microbiol 73, 006083 (2023); https://doi.org/10.1099/ijsem.0.006083
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