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Volume 73, Issue 10

sp. nov., isolated from the gut of No Access

Abstract

A novel Gram-stain-positive bacterium, designated NB10, was isolated from the gut of . The isolate was rod-shaped, aerobic, non-motile and non-spore-forming. Colonies of strain NB10 were light yellow, circular and smooth. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that the isolate was related to the genu. Its closest relatives were T14 (97.8 % 16S rRNA gene sequence similarity), CC-MF41 (97.0%) and M1-8 (96.4%). The DNA G+C content of strain NB10 was 68.8 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization values among strain NB10 and the selected species were ≤83.8 % (ANI-), 87.6 % (ANI-MUMmer) and 29.6%, which were below the recommended cutoff values for species delineation. The predominant cellular fatty acids were anteiso-C (39.0%), anteiso-C (35.5%) and iso-C (17.0%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and glycolipids. The predominant respiratory quinones were MK-11 and MK-10. The cell wall amino acids were Gly, Glu, Tr, Ala, and DAB. Based on these phylogenetic and phenotypic results, strain NB10 can be clearly distinguished from all of the recognized species of the genus and is considered to represent a novel species of that genus. The name sp. nov. is proposed, with the type strain NB10 (=MCCC 1K07072=KCTC 49728).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Leucobacter tenebrionis and taxonomy
Funding
This study was supported by the:
  • Inner Mongolia Natural Science Fund Project (Award 2020MS03057)
    • Principle Award Recipient: XiaoshuanBai
  • Inner Mongolia Science Technology project plan (Award 2020GG0039)
    • Principle Award Recipient: BoYuan
© 2023 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.006081
2023-10-12
2024-05-08
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Leucobacter tenebrionis sp. nov., isolated from the gut of Tenebrio molitor
Int J Syst Evol Microbiol 73, 006081 (2023); https://doi.org/10.1099/ijsem.0.006081
/content/journal/ijsem/10.1099/ijsem.0.006081
/content/journal/ijsem/10.1099/ijsem.0.006081
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