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Abstract
Two Gram-stain-negative, non-motile, non-spore-forming, strictly aerobic and rod-shaped bacterial strains, CMA-7T and CAA-3, were isolated from surface seawater samples collected from the western Pacific Ocean. Phylogeny of 16S rRNA gene sequences indicated they were related to the genera Galbibacter and Joostella and shared 95.1, 90.9 and 90.8% sequence similarity with G. mesophilus Mok-17T, J. marina DSM 19592T and G. marinus ck-I2-15T, respectively. Phylogenomic analysis showed that the two strains, together with the members of the genera Galbibacter and Joostella , formed a monophyletic clade that could also be considered a monophyletic taxon. This distinctiveness was supported by amino acid identity and percentage of conserved proteins indices, phenotypic and chemotaxonomic characteristics and comparative genomics analysis. Digital DNA‒DNA hybridization values and average nucleotide identities between the two strains and their closest relatives were 18.0–20.8 % and 77.7–79.3 %, respectively. The principal fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, Summed Feature 3 (C16 : 1 ω7c/C16 : 1 ω6c or C16 : 1 ω6c/C16 : 1 ω7c), Summed Feature 9 (iso-C17 : 1 ω9c or C16 : 0 10-methyl), and C15 : 0 3-OH. The predominant respiratory quinone was MK-6. The polar lipids were phosphatidylethanolamine, aminolipid, aminophospholipid, phospholipid, phosphoglycolipid, glycolipid and unknown polar lipid. The genomic DNA G+C content of strains CMA-7T and CAA-3 was both 38.4 mol%. Genomic analysis indicated they have the potential to degrade cellulose and chitin. Based on the polyphasic evidence presented in this study, the two strains represent a novel species within the genus Galbibacter , for which the name Galbibacter pacificus sp. nov. is proposed. The type strain is CMA-7T (=MCCC M28999T = KCTC 92588T). Moreover, the transfer of Joostella marina to the genus Galbibacter as Galbibacter orientalis nom. nov. (type strain En5T = KCTC 12518T = DSM 19592T=CGMCC 1.6973T) is also proposed.
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Funding
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National Science and Technology Fundamental Resources Investigation Program of China
(Award 2021FY100900)
- Principle Award Recipient: QiliangLai
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National Natural Science Foundation of China
(Award 42030412)
- Principle Award Recipient: ZongzeShao
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National Natural Science Foundation of China
(Award 41976107)
- Principle Award Recipient: ChunmingDong
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China Ocean Mineral Resource R&D Association program
(Award DY135-B2-01)
- Principle Award Recipient: ZongzeShao
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China Ocean Mineral Resource R&D Association program
(Award DY-XZ-04)
- Principle Award Recipient: ZongzeShao
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National Natural Science Foundation of Fujian Province, China
(Award 2021J01508)
- Principle Award Recipient: ChunmingDong
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Scientific Research Foundation of Third Institute of Oceanography, MNR
(Award 2019021)
- Principle Award Recipient: ZongzeShao
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Scientific Research Foundation of Third Institute of Oceanography, MNR
(Award 2020009)
- Principle Award Recipient: ChunmingDong