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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

sp. nov., isolated from surface seawater of the western Pacific Ocean and transfer of to the genus as nom. nov. and emended description of the genus No Access

Abstract

Two Gram-stain-negative, non-motile, non-spore-forming, strictly aerobic and rod-shaped bacterial strains, CMA-7 and CAA-3, were isolated from surface seawater samples collected from the western Pacific Ocean. Phylogeny of 16S rRNA gene sequences indicated they were related to the genera and and shared 95.1, 90.9 and 90.8% sequence similarity with Mok-17, DSM 19592 and ck-I2-15, respectively. Phylogenomic analysis showed that the two strains, together with the members of the genera and , formed a monophyletic clade that could also be considered a monophyletic taxon. This distinctiveness was supported by amino acid identity and percentage of conserved proteins indices, phenotypic and chemotaxonomic characteristics and comparative genomics analysis. Digital DNA‒DNA hybridization values and average nucleotide identities between the two strains and their closest relatives were 18.0–20.8 % and 77.7–79.3 %, respectively. The principal fatty acids were iso-C, iso-C 3-OH, iso-C G, Summed Feature 3 (C 7/C c or C cC1 c), Summed Feature 9 (iso-C c or C 10-methyl), and C 3-OH. The predominant respiratory quinone was MK-6. The polar lipids were phosphatidylethanolamine, aminolipid, aminophospholipid, phospholipid, phosphoglycolipid, glycolipid and unknown polar lipid. The genomic DNA G+C content of strains CMA-7 and CAA-3 was both 38.4 mol%. Genomic analysis indicated they have the potential to degrade cellulose and chitin. Based on the polyphasic evidence presented in this study, the two strains represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CMA-7 (=MCCC M28999 = KCTC 92588). Moreover, the transfer of to the genus as nom. nov. (type strain En5 = KCTC 12518 = DSM 19592=CGMCC 1.6973) is also proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Galbibacter orientalis nom. nov , Galbibacter pacificus sp. nov , ocean surface seawater , polyphasic taxonomy and west Pacific Ocean
Funding
This study was supported by the:
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: QiliangLai
  • National Natural Science Foundation of China (Award 42030412)
    • Principle Award Recipient: ZongzeShao
  • National Natural Science Foundation of China (Award 41976107)
    • Principle Award Recipient: ChunmingDong
  • China Ocean Mineral Resource R&D Association program (Award DY135-B2-01)
    • Principle Award Recipient: ZongzeShao
  • China Ocean Mineral Resource R&D Association program (Award DY-XZ-04)
    • Principle Award Recipient: ZongzeShao
  • National Natural Science Foundation of Fujian Province, China (Award 2021J01508)
    • Principle Award Recipient: ChunmingDong
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award 2019021)
    • Principle Award Recipient: ZongzeShao
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (Award 2020009)
    • Principle Award Recipient: ChunmingDong
© 2023 The Authors
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Galbibacter pacificus sp. nov., isolated from surface seawater of the western Pacific Ocean and transfer of Joostella marina to the genus Galbibacter as Galbibacter orientalis nom. nov. and emended description of the genus Galbibacter
Int J Syst Evol Microbiol 73, 006078 (2023); https://doi.org/10.1099/ijsem.0.006078
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