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Volume 73, Issue 10

sp. nov., the first non-thermotolerant methanotroph of the genus , from a rice field No Access

Abstract

Strain 16-5, a mesophilic methanotroph of the genus , was isolated from rice field soil sampled in Chungcheong Province, Republic of Korea. Strain 16-5 had both particulate and soluble methane monooxygenases and could only grow on methane and methanol as electron donors. Strain 16-5 cells are Gram-negative, white to light tan in color, non-motile, non-flagellated, diplococcoid to cocci, and have the typical type I intracytoplasmic membrane system. Strain 16-5 grew at 18–38 °C (optimum, 27 °C) and at pH 5.0–8.0 (optimum, pH 6.5–7.0). C ω7 (38.8%), C 5 (18.8%), C 6 (16.8%) and C (16.9%) were the major fatty acids, and phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid were the major polar lipids. The main respiratory quinone was methylene-ubiquinone-8. Strain 16-5 displayed the highest 16S rRNA gene sequence similarities to other taxonomically recognized members of the genus , i.e. Texas (98.62%) and IM1 (98.49 %), which were its closest relatives. It did, however, differ from all other taxonomically described species due to some phenotypic differences, most notably its inability to grow at temperatures above 38 °C, where other species thrive. Its 4.34 Mbp-sized genome has a DNA G+C content of 62.47 mol%, and multiple genome-based properties such as average nucleotide identity and digital DNA-DNA hybridization value distanced it from its closest relatives. Based on the data presented above, this strain represents the first non-thermotolerant species of the genus . The name sp. nov. is proposed, and 16-5 (=JCM 35359=KCTC 82050) is the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): mesophilic , methane , methanotroph , Methylococcus and thermotolerant
Funding
This study was supported by the:
  • NRF KOREA (Award NIBR202325101)
    • Principle Award Recipient: Sung-KeunRhee
  • NRF KOREA (Award PJ01700703)
    • Principle Award Recipient: Sung-KeunRhee
  • NRF KOREA (Award 2021R1A2C3004015)
    • Principle Award Recipient: Sung-KeunRhee
© 2023 The Authors
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2023-10-12
2024-05-08
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Methylococcus mesophilus sp. nov., the first non-thermotolerant methanotroph of the genus Methylococcus, from a rice field
Int J Syst Evol Microbiol 73, 006077 (2023); https://doi.org/10.1099/ijsem.0.006077
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