1887

Abstract

Forty-eight strains were isolated from soils and sediments in Japan. Among them, six representative strains, designated W79, W786, Red222, Red802, Red803, and Red804, were subjected to the taxonomic classification. These six strains are Gram-stain-negative, non-spore-forming, rod-shaped, and facultative anaerobic bacterium that can reduce ferric iron. Phylogenetic and phylogenomic trees based on 16S rRNA genes and multiple single-copy gene sequences showed that strains Red222, Red802, Red803, and Red804 formed a cluster with the type strains of species, but strains W79 and W786 created an independent cluster from any other type strains. The former four strains shared 97.95–99.08% similarities of 16S rRNA gene sequence with the type strains of the genus , whereas the latter two strains 94.86–95.49% similarities. The average amino acid identity of strains W79 and W786 were <63 % to any other type strains, which were below the genus delineation thresholds. Moreover, colonies of these two strains were white, while those of the other four isolated strains were reddish-yellow as well as the type strain H-5. Although the known type strains of species have been reported to be non-motile, five strains (W79, W786, Red222, Red803, and Red804) except for strain Red802 displayed motility. Furthermore, multiple genomic, phylogenetic, and phenotypic features supported the discrimination between these isolated strains. Based on the study evidence, we propose these six isolates as novel members within the ///, comprising two novel species of a novel genus, gen. nov., sp. nov., and sp. nov., and four novel species of the genus sp. nov., sp. nov., sp. nov., and sp. nov.

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award JP20K15423)
    • Principle Award Recipient: YokoMasuda
  • Japan Society for the Promotion of Science (Award JP20H05679)
    • Principle Award Recipient: HideomiItoh
  • Japan Society for the Promotion of Science (Award JP20H00409)
    • Principle Award Recipient: HideomiItoh
  • The Canon Foundation
    • Principle Award Recipient: HideomiItoh
  • Institute for Fermentation, Osaka (Award G-2021-1-028)
    • Principle Award Recipient: HideomiItoh
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/content/journal/ijsem/10.1099/ijsem.0.006073
2023-09-06
2024-06-13
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