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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

Identification of sp. nov. and sp. nov., two novel members of the genus Open Access

Abstract

Six psychrotolerant, Gram-stain-negative, aerobic bacterial strains, designated as LB1P51, LB2P87, LB2P84, LB3P48, LB3R18 and XS2P67, were isolated from glaciers on the Tibetan Plateau, PR China. The results of 16S rRNA gene analysis confirmed their classification within the genus . Strain LB2P87 displayed the highest sequence similarity to 0533 (98.18 %), while strain LB1P51 exhibited the highest sequence similarity to CGMCC 1.5380 (98.15 %). Strains LB2P87 and LB1P51 had genome sizes of 3.8 and 3.9 Mb, respectively, with DNA G+C contents of 34.2 and 34.1 %, respectively. Pairwise average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) calculations revealed that these strains represented two distinct species within the genus . The results of phylogenomic analysis using 606 core genes indicated that the six strains formed a distinct clade and were most closely related to CGMCC 1.5380. The ANI and dDDH values between the two species and other members of the genus were below 90.3 and 40.1 %, respectively. Genome relatedness, the results of phylogenomic analysis and phenotypic characteristics collectively support the proposal of two novel species of the genus : sp. nov. (LB1P51 = CGMCC 1.11237 = NBRC 114813) and sp. nov. (LB2P87 = CGMCC 1.11249 = NBRC 114814).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cold-adapted bacteria , Flavobacterium and glacier
Funding
This study was supported by the:
  • Second Tibetan Plateau Scientific Expedition and Research Program (Award 2021QZKK0100)
    • Principle Award Recipient: JianYe
  • National Natural Science Foundation of China (Award 32170007)
    • Principle Award Recipient: QingLiu
© 2023 Not applicable
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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Identification of Flavobacterium algoritolerans sp. nov. and Flavobacterium yafengii sp. nov., two novel members of the genus Flavobacterium
Int J Syst Evol Microbiol 73, 006072 (2023); https://doi.org/10.1099/ijsem.0.006072
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