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Volume 73, Issue 9

sp. nov., isolated from the Western Pacific Ocean No Access

Abstract

A novel Gram-stain-negative, facultatively anaerobic and heterotrophic bacterium, designated strain ZH257, was isolated from enrichment samples incubated on the seamount floor of the Western Pacific Ocean. Cells were rod-shaped, oxidase- and catalase- positive, and motile by means of polar flagella. Strain ZH257 grew at 4–37 °C (optimum, 28–32 °C), pH 6.0–9.0 (optimum, pH 7.0) and with 2.0–9.0 % (w/v) NaCl (optimum, 3.0–4.0 %). Strain ZH257 was most closely related to members of the genus , sharing 99.13, 98.27 and 96.89 % 16S rRNA gene sequence identities with GDMCC 1.2988, DSM 23566 and DSM 25627, respectively. The DNA G+C content was 59.2 mol%. The estimated average nucleotide identity and digital DNA–DNA hybridization values between strain ZH257 and its closely related species were 79.61–93.04 % and 23.10–50.20 %, respectively. Strain ZH257 harboured complete denitrification and nitrate assimilation pathways. Strain ZH257 contained summed feature 8 (C 7 and/or C 6) as major fatty acids (>5 %), and Q-10 as the major respiratory quinone. The polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and four unidentified lipids. The combined phenotypic, genotypic and chemotaxonomic data showed that strain ZH257 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain ZH257 (=MCCC M29024=KACC 23147).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep-sea , Pseudophaeobacter , taxonomy and Western Pacific Ocean
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award No.42030412)
    • Principle Award Recipient: ZongzeShao
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (CN) (Award No. 2019021)
    • Principle Award Recipient: ZongzeShao
  • Scientific Research Foundation of Third Institute of Oceanography, MNR (CN) (Award No. 2019027)
    • Principle Award Recipient: ZongzeShao
© 2023 The Authors
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2023-09-28
2024-05-09
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Pseudophaeobacter profundi sp. nov., isolated from the Western Pacific Ocean
Int J Syst Evol Microbiol 73, 006071 (2023); https://doi.org/10.1099/ijsem.0.006071
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