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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 9

sp. nov., a polyene producer, and sp. nov., two novel actinobacterial species from peat swamp forests in Thailand No Access

Abstract

Two novel actinobacterial strains, designated RB6PN23 and K1PA1, were isolated from peat swamp soil samples in Thailand and characterized using a polyphasic taxonomic approach. The strains were filamentous Gram-stain-positive bacteria containing -diaminopimelic acid in their whole-cell hydrolysates. Phylogenetic analysis of their 16S rRNA gene sequences revealed that strain RB6PN23 was most closely related to (99.1 % sequence similarity) and (98.5%), while strain K1PA1 showed 98.8 and 98.7% sequence similarities to and , respectively. However, the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were below the species-level thresholds (95–96 % ANI and 70 % dDDH). The genomes of strains RB6PN23 and K1PA1 were estimated to be 7.88 Mbp and 7.39 Mbp in size, respectively, with DNA G+C contents of 70.2 and 73.2 mol%. Moreover, strains RB6PN23 and K1PA1 encode 37 and 24 putative biosynthetic gene clusters, respectively, and analysis revealed that these new species have a high potential to produce unique natural products. Genotypic and phenotypic characteristics confirmed that strains RB6PN23 and K1PA1 represented two novel species in the genus . The names proposed for these strains are sp. nov. (type strain RB6PN23=TBRC 17040=NBRC 116113) and sp. nov. (type strain K1PA1=TBRC 17041=NBRC 116114). Additionally, a giant linear polyene compound, neotetrafibricin A, exhibiting antifungal activity in strain RB6PN23, was identified through HPLC and quadrupole time-of-flight MS analysis. The crude extract from the culture broth of strain RB6PN23 exhibited strong antifungal activity against , and . This finding suggests that strain RB6PN23 could be a promising candidate for biological control of fungal diseases.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinomycete , antifungal activity , polyene , polyphasic taxonomy and Streptomyces
Funding
This study was supported by the:
  • Royal Golden Jubilee (RGJ) Ph.D. Programme (Award PHD/0131/2560)
    • Principle Award Recipient: ChollachaiKlaysubun
© 2023 The Authors
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2023-09-28
2024-05-09
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Streptomyces silvisoli sp. nov., a polyene producer, and Streptomyces tropicalis sp. nov., two novel actinobacterial species from peat swamp forests in Thailand
Int J Syst Evol Microbiol 73, 006063 (2023); https://doi.org/10.1099/ijsem.0.006063
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