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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 9

sp. nov. and sp. nov., two novel actinomycetes isolated from No Access

Abstract

Two novel Gram-positive bacteria designated as strains STR2 and STR3 were isolated from the rhizosphere of a sample collected from Goyang-si, Republic of Korea. Strains STR2 and STR3 were aerobic, rod shaped, non-sporulated, catalase negative, oxidase negative and non-motile bacteria. They grew at 15–37 °C (optimum, 25–30 °C), at pH 6.0–11.0 (optimum, pH 7.0) and in the presence of 0–2% NaCl (optimum, 0 %, w/v). The chemotaxonomic and morphological characteristics of the novel strains were consistent with those of the members of . The phylogenetic analysis of the 16S rRNA gene sequences revealed that STR2 was closely related to YIM A1136 (99.3 %) and Y4 (99.1 %), and STR3 was closely related to DSM 22017 (99.0 %), G10 (98.8 %) and HFW-21 (98.7 %). The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values of STR2 and STR3 with the most closely related strains that have publicly available whole genomes were 83.1–89.8 %, 80.9–89.6% and 26.2–39.1 %, respectively. The cell-wall peptidoglycan of strain STR2 and STR3 contained -diaminopimelic acid as the diagnostic amino acid. The major fatty acids in STR2 and STR3 were iso-C and C ω8, and the predominant quinone was MK-8(H). Their polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and other polar lipids. The draft genome sequences showed that the genomic DNA G+C content of STR2 and STR3 were both 72.2 mol%. Physiological and biochemical tests and 16S rRNA sequence analysis clearly revealed that STR2 and STR3 could represent novel species. Their proposed names were as follows: sp. nov. for strain STR2 (=KACC 22784=TBRC 16336) and sp. nov. for strain STR3 (= KACC 22785=TBRC 16337).

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Keyword(s): Nocardioidaceae , Nocardioides pini , Nocardioides pinisoli and Pinus densiflora
© 2023 The Authors
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2023-09-27
2024-05-08
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Nocardioides pini sp. nov. and Nocardioides pinisoli sp. nov., two novel actinomycetes isolated from Pinus densiflora
Int J Syst Evol Microbiol 73, 006062 (2023); https://doi.org/10.1099/ijsem.0.006062
/content/journal/ijsem/10.1099/ijsem.0.006062
/content/journal/ijsem/10.1099/ijsem.0.006062
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