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Volume 73, Issue 9

sp. nov., isolated from a rice field No Access

Abstract

A Gram-stain-negative, aerobic, short rod-shaped and motile novel bacterial strain, designated MAHUQ-71, was isolated from the soil of a rice field. The colonies were observed to be milky yellow-coloured, smooth, spherical and 0.1–0.4 mm in diameter when grown on Reasoner's 2A agar medium for 2 days. Strain MAHUQ-71 was found to be able to grow at 15–37 °C, pH 5.0–10.0 and with 0–3.0 % NaCl (w/v). The strain was found to be positive for the catalase test, but negative for the oxidase test. The strain was positive for hydrolysis of aesculin and Tween 20. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus and to be closely related to MAH-6 (98.5 % sequence similarity), B2-7 (98.4 %) and SY-6 (96.6 %). Strain MAHUQ-71 has a draft genome size of 4 255 278 bp (10 contigs), annotated with 4098 protein-coding genes, 47 tRNA and three rRNA genes. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain MAHUQ-71 and the closest type strain MAH-6 were in the range of 85.6 and 30.6 %, respectively. The genomic DNA G+C content was determined to be 66.7 mol%. The predominant isoprenoid quinone was ubiquinone 10. The major fatty acids were identified as summed feature 8 (C 7 and/or C 6), C and C 2OH. The main polar lipids were phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol and sphingoglycolipid. On the basis of dDDH and ANI values, as well as the results of genotypic, chemotaxonomic and physiological analyses, strain MAHUQ-71 represents a novel species within the genus , for which the name sp. nov. is proposed, with MAHUQ-71 (=KACC 22252=CGMCC 1.19065) as the type strain.

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Keyword(s): digital DNA–DNA hybridization , genome sequence , rice field and Sphingomonas oryzagri
© 2023 The Authors
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2023-09-27
2024-05-08
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Sphingomonas oryzagri sp. nov., isolated from a rice field
Int J Syst Evol Microbiol 73, 006061 (2023); https://doi.org/10.1099/ijsem.0.006061
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