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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 9

sp. nov., a facultative anaerobic bacterium belonging to the family isolated from marine sediment in the East China Sea No Access

Abstract

A facultative anaerobic, Gram-strain-negative, rod-shaped bacterium (strain NBU2970) was isolated by using modified ichip cultivation from a marine sediment sample collected from Meishan Island in the East China Sea. Strain NBU2970 grew optimally at 37 °C, with a NaCl concentration of 2.0 % (w/v) and at pH 7.0. The 16S rRNA gene sequence analyses revealed that strain NBU2970 represents a novel species with the genus , sharing highest sequence identities with BB-My12 (96.1 %), SCR12 (96.0 %), 105 (96.0 %) and B1 (95.6 %). Phylogenetic analyses also indicated that strain NBU2970 clustered with the genus and was closely related to BB-My12 and B1. The draft genome sequence of strain NBU2970 was composed of six contigs with a size of 3.2 Mbp, containing 3045 protein-coding genes and 38 RNA genes. The DNA G+C content was 43.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain NBU2970 and related species of the genus were well below the threshold limit for prokaryotic species delineation. The major cellular fatty acids were iso-C, iso-C G and iso-C 3-OH. The only respiratory quinone was MK-6. The major polar lipid was phosphatidylethanolamine. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2970 is considered to be a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is NBU2970 (=KCTC 82915=MCCC 1K06394).

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Keyword(s): marine sediment , Muricauda meishanensis and taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Zhejiang Province (Award LGF22C010001)
  • National Natural Science Foundation of China (Award 32100001)
    • Principle Award Recipient: ZhangWeiyan
© 2023 The Authors
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2023-09-26
2025-04-19
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/content/journal/ijsem/10.1099/ijsem.0.006059
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Muricauda meishanensis sp. nov., a facultative anaerobic bacterium belonging to the family Flavobacteriaceae isolated from marine sediment in the East China Sea
Int J Syst Evol Microbiol 73, 006059 (2023); https://doi.org/10.1099/ijsem.0.006059
/content/journal/ijsem/10.1099/ijsem.0.006059
/content/journal/ijsem/10.1099/ijsem.0.006059
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