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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 9

sp. nov. and sp. nov., isolated from phycosphere of marine algae No Access

Abstract

Two Gram-negative, moderately halophilic, and motile rod bacteria, strains G2-23 and J2-29, showing catalase- and oxidase-positive activities were isolated from species of the marine algae and , respectively. Both strains optimally grew at 30 °C, pH 7.0 and 2% (w/v) NaCl. Both strains contained ubiquinone-10 as the sole isoprenoid quinone. Strain G2-23 contained summed feature 8 (C 7 and/or C 6), C and summed feature 3 (iso-C 2-OH and/or C cωc) as major cellular fatty acids, and phosphatidylethanolamine (PE), phosphatidyl--monomethylethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol and an unidentified phospholipid (PL) as major polar lipids. Strain J2-29 contained summed feature 8, C 7 11-methyl and C as major cellular fatty acids and PE, PME, PG and PL as major polar lipids. The genomic DNA G+C contents of strains G2-23 and J2-29 were 59.5 and 62.2 mol%, respectively. Both strains shared 97.9 % 16S rRNA gene sequence similarity, 79.8 % average nucleotide identity (ANI) and 22.8 % digital DNA–DNA hybridization (dDDH) values, indicating that they represent different species. Phylogenetic and phylogenomic analyses by 16S rRNA gene and genome sequences, respectively, revealed that strains G2-23 and J2-29 formed different phylogenic lineages within the genus . ANI and dDDH values between strains G2-23 and J2-29 and other type strains were less than 79.0 and 22.1% and 80.5 and 23.3 %, respectively, suggesting that they represent novel species of the genus . In summary, based on their phenotypic, chemotaxonomic and molecular properties, strains G2-23 and J2-29 represent two different novel species of the genus , for which the names sp. nov. (G2-23=KACC 22714=JCM 35548) and sp. nov. (J2-29=KACC 22715=JCM 35549), respectively, are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Hoeflea algicola , Hoeflea ulvae , marine algae , new taxa and Pseudomonadota
Funding
This study was supported by the:
  • National Institute of Biological Resources (KR) (Award NIBR No. 2022-02-001)
    • Principle Award Recipient: CheOk Jeon
  • Chung-Ang University (Award 2023)
    • Principle Award Recipient: CheOk Jeon
© 2023 The Authors
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2023-09-27
2024-05-08
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Hoeflea algicola sp. nov. and Hoeflea ulvae sp. nov., isolated from phycosphere of marine algae
Int J Syst Evol Microbiol 73, 006058 (2023); https://doi.org/10.1099/ijsem.0.006058
/content/journal/ijsem/10.1099/ijsem.0.006058
/content/journal/ijsem/10.1099/ijsem.0.006058
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