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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

gen. nov., sp. nov., a novel member of the family isolated from stony coral in the South China Sea No Access

Abstract

Two novel filamentous bacteria, designated as IB182353 and IB182357, were isolated from stony coral of the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains IB182353 and IB182357 were closely related to DSM 45707 (with 93.4 and 93.5% similarity, respectively). The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization results showed that the pairwise similarities between isolate IB182353 and the other recognized species were less than 68.9, 60.5 and 21.1 %, respectively. Both strains produced aerial and substrate mycelia, grew optimally at 25–30 °C, pH 8.0–9.0 and with 2–3 % (w/v) NaCl. The cell-wall peptidoglycan type was -DAP and the whole-cell hydrolysates contained ribose. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid and three unidentified phospholipids. The genomic DNA G+C content was 39.5 mol%. Strain IB182353 was distinguishable from its related type strains by the contents of two fatty acids, iso-C and iso-C 10. Based on polyphasic taxonomic characterization, we propose that strains IB182353 and IB182357 represent a novel genus and species within the family , for which the name gen. nov. sp. nov. is proposed. The type strain is IB182353 (=MCCC 1K04631=JCM 34206).

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene , Polycladospora coralii gen. nov., sp. nov and polyphasic taxonomy
Funding
This study was supported by the:
  • Special Fund for Agro-scientific Research in the Public Interest (Award 1630052019014)
    • Principle Award Recipient: Yonghua Hu
  • Department of Agriculture and Rural Affairs of Zhejiang Province (Award NHYYSWZZZYKZX2020)
    • Principle Award Recipient: Yonghua Hu
© 2023 The Authors
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2023-10-04
2024-05-08
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Polycladospora coralii gen. nov., sp. nov., a novel member of the family Thermoactinomycetaceae isolated from stony coral in the South China Sea
Int J Syst Evol Microbiol 73, 006055 (2023); https://doi.org/10.1099/ijsem.0.006055
/content/journal/ijsem/10.1099/ijsem.0.006055
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