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Volume 73, Issue 10

sp. nov., a novel member of the genus , isolated from the West Pacific Ocean No Access

Abstract

Two Gram-stain-negative, aerobic, non-motile and short-rod-shaped bacteria, designated as strains GL-53 and GL-15-2-5, were isolated from the seamount area of the West Pacific Ocean and identified using a polyphasic taxonomic approach. The growth of strains GL-53ᵀ and GL-15-2-5 occurred at pH 5.5–10.0, 4–40 °C (optimum at 28 °C) and 0–10.0 % NaCl concentrations (optimum at 0–5.0 %). On the basis of 16S rRNA gene sequence analysis, strains GL-53ᵀ and GL-15-2-5 exhibited the highest similarity to YQF-2 (98.4 %), followed by KMM 1406 (98.1 %), E407-8 (97.4 %), H29 (97.4 %), E48 (97.2 %) and SW-353 (97.2 %). Phylogenetic analysis revealed that the isolates were affiliated with the genus and represented an independent lineage. The major fatty acids were summed feature 3 (C 7 and/or C 6), C and summed feature 8 (C 7 and/or C 6). The sole isoprenoid quinone was ubiquinone 8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid (and one unidentified glycolipid. The DNA G+C content was 48.5 mol%. The average nucleotide identity, average amino acid identity and DNA–DNA hybridization values among the genomes of strain GL-53ᵀ and the related strains in the genus were 75.5–90.1 %, 67.5–93.9 % and 21.4–41.4 %, respectively. Based on their phenotypic, chemotaxonomic and genotypic properties, the two strains were identified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GL-53 (=KCTC 82651=MCCC M20598).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chromatiaceae , Chromatiales , genome analysis , phylogenetic analysis and Rheinheimera
Funding
This study was supported by the:
  • the Scientific Research Fund of Second Institute of Oceanography, MNR (Award JG1521)
    • Principle Award Recipient: Chen-HuaHan
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2021FY100900)
    • Principle Award Recipient: PengZhou
© 2023 The Authors
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2023-10-20
2024-05-08
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Rheinheimera oceanensis sp. nov., a novel member of the genus Rheinheimera, isolated from the West Pacific Ocean
Int J Syst Evol Microbiol 73, 006054 (2023); https://doi.org/10.1099/ijsem.0.006054
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