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Volume 73, Issue 9

sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse Open Access

Abstract

A novel, anaerobic, Gram-stain-positive coccoid strain, CBA3646, was isolated from the faeces of a thoroughbred racehorse. Phylogenetic analysis based on 16S rRNA gene sequencing yielded results indicative of CBA3646 representing a member of the genus , with the species most closely related to it being DSM 20463, with a similarity of 94.79 %. DNA–DNA relatedness and average nucleotide identity values between CBA3646 and DSM 20463 were 21.4 and 67.6 %, respectively. CBA3646 has a circular chromosomal genome of 1 709 189 bp (45.5 mol% DNA G+C content), containing 1652 genes in total, 1584 predicted protein-coding genes, 3 complete rRNA loci and 47 tRNA genes. The cells were non-motile diplococci, catalase-positive and oxidase-negative. Growth of CBA3646 was observed at 20–40 °C (optimal temperature, 35 °C) and in the presence of 0–4 % (w/v) NaCl (optimum concentration, 1 %). The major fatty acids (>10 %) of CBA3646 were C, Cω9 and Cω9 dimethyl acetal, with its major polar lipids being diphosphatidylglycerol and phosphatidylglycerol. The elucidated phylogenetic, physiological, chemotaxonomic and molecular properties are indicative of strain CBA3646 representing a novel species of the genus , or which the name sp. nov. is proposed. The type strain is CBA3646 (= KACC 22890 = JCM 35845).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): horse faeces , novel taxon , Peptoniphilus equinus , taxonomy and thoroughbred racehorse
Funding
This study was supported by the:
  • World Institute of Kimchi (Award KE2301-1-1)
    • Principle Award Recipient: TaeWoong Whon
  • National Research Foundation of Korea (NRF) (Award 2021R1C1C1013859)
    • Principle Award Recipient: SeHee Lee
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-09-26
2024-05-08
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Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse
Int J Syst Evol Microbiol 73, 006053 (2023); https://doi.org/10.1099/ijsem.0.006053
/content/journal/ijsem/10.1099/ijsem.0.006053
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