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Abstract

Two rod-shaped, facultative anaerobic, Gram-stain-positive lactic acid bacteria were isolated from corn silage and grass silage. They were characterized using a polyphasic approach and designated as HO 1656 and HO 0673. Analysis of 16S rRNA gene sequence of both strains indicated that they belong to the group. The most closely related species, DSM 20011 and DSM 20178, have digital DNA–DNA hybridization (dDDH) values of 63.9 and 53.4%, respectively, with the novel strains. In contrast, the dDDH value between strains HO 1656 and HO 0673 is 99.3 %, clearly showing that these two isolated strains belong to the same species. According to analysis of the housekeeping genes (, and ), both strains form a distinct cluster within the group. Strains HO 0673 and HO 1656 could produce acid from -arabinose, adonitol, ribose, rhamnose, dulcitol, sorbitol, turanose, -fucose and -arabitol, unlike their nearest phylogenetic neighbour DSM 20011. The major cellular fatty acids of both strains are C and C ω9. The G+C content of the genomic DNA of both strains is 48.0 mol%. Thus, strains HO 1656 and HO 0673 represent a novel species based on their chemotaxonomic, phenotypic and phylogenetic characteristics. The name sp. nov. is proposed with the type strain HO 1656 (=DSM 115425=NCIMB 15466).

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2023-10-03
2024-11-08
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