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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 10

Characterization of two novel Fe(III)-reducing and electrogenic bacteria, sp. nov. and sp. nov., isolated from mangrove sediment No Access

Abstract

Three novel strains in the genus , designated A3A, C31 and C32, were isolated from mangrove sediment samples. They were facultative anaerobic, Gram-stain-negative, rod-shaped, flagellum-harbouring, oxidase- and catalase-positive, electrogenic and capable of using Fe(III) as an electron acceptor during anaerobic growth. Results of phylogenetic analysis based on 16S rRNA gene and genomic sequences revealed that the strains should be assigned to the genus . The 16S rRNA gene similarity, average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the isolates and their closely related species were below the respective cut-off values for species differentiation. The 16S rRNA gene similarity, ANI and dDDH values between strains C31 and C32 were 99.7, 99.9 and 99.9 %, respectively, indicating that they should belong to the same genospecies. Based on polyphasic taxonomic approach, two novel species are proposed, sp. nov. with type strain A3A (GDMCC 1.2732=JCM 34899) and sp. nov. with type strain C31 (GDMCC 1.2736=JCM 34902).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): electrogenesis , ferric iron reduction , novel species , polyphasic taxonomic approach and Shewanella
Funding
This study was supported by the:
  • Natural Science Foundation of Guangdong Province (Award 2023A1515030281)
    • Principle Award Recipient: GuiqinYang
  • Natural Science Foundation of Guangdong Province (Award 2022A1515011734)
    • Principle Award Recipient: LiZhuang
  • National Natural Science Foundation of China (Award 42077211)
    • Principle Award Recipient: LiZhuang
© 2023 The Authors
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2023-10-12
2024-05-08
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Characterization of two novel Fe(III)-reducing and electrogenic bacteria, Shewanella ferrihydritica sp. nov. and Shewanella electrica sp. nov., isolated from mangrove sediment
Int J Syst Evol Microbiol 73, 006044 (2023); https://doi.org/10.1099/ijsem.0.006044
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