1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 9

sp. nov., sp. nov. and sp. nov., isolated from freshwater at Jucheon River, and emended description of comb. nov. No Access

Abstract

Three Gram-stain-negative, facultatively anaerobic, rod-shaped, catalase-positive, oxidase-negative bacterial strains were designated as hw1, hw8 and hw3. Strains hw1, hw8 and hw3 grew at 15–28 °C (optimum, 25 °C), 15–35 °C (optimum, 30 °C) and 4–28 °C (optimum, 20 °C), respectively, and at pH 7.0–12.0 (optimum, pH 9.0), pH 6.0–11.0 (optimum, pH 9.0) and 5.0–12.0 (optimum, pH 7.0), respectively. Additionally, strains hw1 and hw8 only grew when the NaCl concentration was 0 %, while strain hw3 grew at between 0 and 0.5 % (w/v; optimum, 0 %). The average nucleotide identity (ANI) values between strains hw1, hw8 and the type strains ranged from 73.8 to 84.2 %, while the digital DNA–DNA hybridization (dDDH) values ranged from 19.7 to 27.5 %. The ANI values between strain hw3 and the type strains ranged from 78.7 to 80.7 %, while dDDH values ranged from 22.3 to 23.0 %. The draft genomes of strains hw1, hw8 and hw3 consisted of 5.5, 4.4 and 5.9 Mbp, with DNA G+C contents of 61.7, 61.8 and 66.0 mol%, respectively. The results of the dDDH, ANI, phylogenetic, biochemical and physiological analyses indicated that the novel strains were distinct from other members of their genera. Thus, we proposed the names sp. nov. (type strain hw1= KACC 22887= TBRC 16613), sp. nov. (type strain hw8= KACC 22885= TBRC 16614) and sp. nov. (type strain hw3= KACC 22886= TBRC 16615).

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Keyword(s): average nucleotide identity , digital DNA–DNA hybridization , draft genome , freshwater , Janthinobacterium and Roseateles
© 2023 The Authors
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2023-09-26
2024-05-08
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Roseateles albus sp. nov., Roseateles koreensis sp. nov. and Janthinobacterium fluminis sp. nov., isolated from freshwater at Jucheon River, and emended description of Roseateles aquaticus comb. nov.
Int J Syst Evol Microbiol 73, 006043 (2023); https://doi.org/10.1099/ijsem.0.006043
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