1887

Abstract

A Gram-stain-negative, aerobic and non-motile rods strain, designated as strain 2012CJ39-3, was isolated from a marine sponge, , collected on Chuja Island, Republic of Korea. Optimal growth of strain 2012CJ39-3 was observed at 25 °C, pH 7.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Strain 2012CJ39-3 contained menaquinone-6 as the respiratory quinone, iso-C G, iso-C, and iso-C 3-OH as the predominant fatty acids, and phosphatidylethanolamine, an unidentified phospholipid, an unidentified glycolipid, three unidentified aminolipids and nine unidentified lipids as major polar lipids. The genomic DNA G+C content was 38.4 mol%. Results of phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealed that strain 2012CJ39-3 formed a distinct phyletic lineage in the genus . Strain 2012CJ39-3 was most closely related to 176CP5-101, 2012CJ35-5, AsT0115, DSM 22638 and SW169 with 96.5, 96.4, 96.3, 95.8 and 95.6 % 16S rRNA gene sequence similarity, respectively. The average nucleotide identity and digital DNA–DNA hybridization values between strain 2012CJ39-3 and 2012CJ35-5, 176CP5-101, AsT0115, DSM 22638 and SW169 were 75.6, 74.2, 78.6, 75.3 and 74.8 % and 27.4, 19.9, 36.3, 24.2 and 18.9 %, respectively. Based on these results, strain 2012CJ39-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2012CJ39-3 (=KACC 22644= LMG 32582). In addition, is reclassified as nom. nov.

Funding
This study was supported by the:
  • Ministry of Education (Award 2021R1I1A3046479)
    • Principle Award Recipient: Jin-SookPark
  • National Marine Biodiversity Institute of Korea
    • Principle Award Recipient: Jin-SookPark
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2023-11-20
2024-07-25
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