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Abstract

Three strains of novel oleaginous yeast species were isolated from soil samples collected in Shiga Prefecture, Japan. The sequences of the internal transcribed spacer (ITS) region and the D1/D2 region of the large subunit (LSU) of the rRNA genes indicated that these novel yeast species are members of the genus . The results of molecular phylogenetic analysis indicated that strains 38–3 and 8s1 were closely related to . They differed by 10 nucleotide substitutions and one gap (1.77 %) in the D1/D2 region of the LSU of the rRNA genes and by 17–18 nucleotide substitutions and 10–11 gaps (5.45–5.85 %) in the ITS region. Strain 51–4 differed from the type strain of the most closely related species, , by 26 nucleotide substitutions (4.46 %) in the D1/D2 region of the LSU of the rRNA genes and by 20 nucleotide substitutions and six gaps (5.42 %) in the ITS region. The names proposed for these previously undescribed species are sp. nov. and sp. nov.

Funding
This study was supported by the:
  • New Energy and Industrial Technology Development Organization
    • Principle Award Recipient: JunOgawa
  • Ryukoku University
    • Principle Award Recipient: JunShima
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-09-20
2024-04-29
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