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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 73, Issue 10

gen. nov., sp. nov., and sp. nov., gen. nov.: novel bacteria from the family isolated from the female genital tract Open Access

Abstract

Four obligately anaerobic Gram-positive bacteria representing one novel genus and two novel species were isolated from the female genital tract. Both novel species, designated UPII 610-J and KA00274, and an additional isolate of each species were characterized utilizing biochemical, genotypic and phylogenetic analyses. All strains were non-motile and non-spore forming, asaccharolytic, non-cellulolytic and indole-negative coccobacilli. Fatty acid methyl ester analysis for UPII 610-J and KA00274 and additional isolates revealed C, C, Cω9c and Cω6,9c to be the major fatty acids for both species. UPII 610-J had a 16S rRNA gene sequence similarity of 99.4 % to an uncultured clone sequence (AY724740) designated as Bacterial Vaginosis Associated Bacterium 2 (BVAB2). KA00274 had a 16S rRNA gene sequence similarity of 96.5 % to UPII 610-J. Whole genomic DNA mol% G+C content was 42.2 and 39.3 % for UPII 610-JT and KA00274, respectively. Phylogenetic analyses indicate these isolates represent a novel genus and two novel species within the family. We propose the names gen. nov., sp. nov., for UPII 610-J representing the type strain of this species (=DSM 112989, =ATCC TSD-274) and gen. nov., sp. nov., for KA00274 representing the type strain of this species (=DSM 112988, =ATCC TSD-275).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacterial vaginosis , BVAB2 , genital tract , human vagina and Oscillospiraceae
Funding
This study was supported by the:
  • National Institutes of Health (Award U19 AI120249)
    • Principal Award Recipient: SharonL. Hillier
  • National Institutes of Health (Award R01 HG005816)
    • Principal Award Recipient: DavidN. Fredricks
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-10-03
2025-12-10

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Amygdalobacter indicium gen. nov., sp. nov., and Amygdalobacter nucleatus sp. nov., gen. nov.: novel bacteria from the family Oscillospiraceae isolated from the female genital tract
Int J Syst Evol Microbiol 73, 006017 (2023); https://doi.org/10.1099/ijsem.0.006017
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