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Volume 73, Issue 10

subsp. subsp. nov., a novel subspecies isolated from the faeces of weaning infants Open Access

Abstract

The species currently comprises four subspecies: subsp. , subsp. , subsp. and subsp. . Recently, several studies on suggested the presence of a separate clade containing four strains isolated from infants and one from rhesus macaque. These strains shared a phylogenetic similarity to subsp. DSM 20210 and subsp. JCM1995 [average nucleotide identity (ANI) of 98.1 %) while showed an ANI of 96.5 % with both subsp. and subsp. . The current work describes five novel additional strains isolated from Bangladeshi weaning infants and demonstrates their common phylogenetic origin with those of the previously proposed separated clade. Based on polyphasic taxonomic approach comprising loci multilocus sequence analysis and whole genome multilocus sequence typing, all ten examined strains have been confirmed as a distinct lineage within the species with subsp. and subsp. as closest subspecies. Interestingly, these strains are present in weaning infants and primates as opposed to their closest relatives which have been typically isolated from pig and calves. These strains, similarly to subsp. , show a common capacity to metabolize the human milk oligosaccharide 3-fucosyllactose. Moreover, they harbour a riboflavin synthesis operon, which differentiate them from their closest subspecies, subsp. and subsp. . Based on the consistent results from genotypical, ecological and phenotypical analyses, a novel subspecies with the name subsp. , with type strain NCC 5000 (=LMG 32752=CCOS 2034), is proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bifidobacterium longum , cgMLST , HMO , MLSA and subspeciation
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-10-18
2024-05-20
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Bifidobacterium longum subsp. iuvenis subsp. nov., a novel subspecies isolated from the faeces of weaning infants
Int J Syst Evol Microbiol 73, 006013 (2023); https://doi.org/10.1099/ijsem.0.006013
/content/journal/ijsem/10.1099/ijsem.0.006013
/content/journal/ijsem/10.1099/ijsem.0.006013
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