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Abstract

A polyphasic taxonomic study was carried out on a Gram-stain-negative and rod-shaped strain, ER-Te-42B-Light, isolated from the tissue of a tube worm, , collected near a deep-sea hydrothermal vent of the Juan de Fuca Ridge in the Pacific Ocean. This bacterium was capable of performing anaerobic respiration using tellurite, tellurate, selenite and orthovanadate as terminal electron acceptors. While facultatively anaerobic, it could aerobically resist tellurite, selenite and orthovanadate up to 2000, 7000 and 10000 µg ml, respectively, reducing each oxide to elemental forms. Nearly complete 16S rRNA gene sequence similarity related the strain to , with 98.8 and 98.7 % similarity to and , respectively. The dominant fatty acids were C and C. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol and MK-7 was the predominant quinone. DNA G+C content was 42.5 mol%. Computation of average nucleotide identity and digital DNA–DNA hybridization values with the closest phylogenetic neighbours of ER-Te-42B-Light revealed genetic divergence at the species level, which was further substantiated by differences in several physiological characteristics. Based on the obtained results, this bacterium was assigned to the genus as a new species with the name sp. nov., type strain ER-Te-42B-Light (=VKM B-3580=DSM 113370).

Funding
This study was supported by the:
  • Wilson Enhancement Fund for Applied Research In Science
    • Principle Award Recipient: JohnA Kyndt
  • University of Manitoba Faculty of Science Scholarship
    • Principle Award Recipient: VladimirYurkov
  • Slippery Rock University Department of Biology
    • Principle Award Recipient: ChrisMaltman
  • Slippery Rock University Faculty Student Research Grant
    • Principle Award Recipient: ChrisMaltman
  • University of Manitoba GETS
    • Principle Award Recipient: VladimirYurkov
  • NSERC
    • Principle Award Recipient: VladimirYurkov
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/content/journal/ijsem/10.1099/ijsem.0.005980
2023-07-21
2024-12-06
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