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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 6

sp. nov. and sp. nov., isolated from paddy field soil No Access

Abstract

Bacterial strains were collected from the soil of a paddy field around Dongguk University in Goyang, Republic of Korea. Two Gram-stain-negative, rod-shaped, aerobic or facultatively anaerobic bacterial strains were designated S5 and Sa. The results of analysis of phylogenetic trees based on 16S rRNA and whole-genome sequences indicated that these two strains represented a member of the genus and a member of the genus , respectively. S5 exhibited 99.22, 98.10 and 97.68 % similarity to HYN0085, YX9 and DSM 19594, respectively. S5 grew at 15–40 °C (optimum, 25 °C), at pH 6.5–12.0 (optimum, pH 9.5) and in the presence of 0–0.5 % (w/v) NaCl (optimum, 0 %). Sa exhibited 99.18 %, 98.36 %, 97.82 % and 97.68 % similarity to ATSB10, DHo, 5HGs31-2 and Gsoil 3046, respectively, and grew at 20–40 °C (optimum, 30 °C), at pH 5.5–11.0 (optimum, pH 8) and in the presence of 0–4.5 % (w/v) NaCl (optimum, 2.5 %). The average nucleotide identity difference values of S5, Sa and the species reference strains were 92.16–93.62 % and 92.71–93.43%, which confirms that the S5 and Sa represent two novel species of the genera and , respectively. The draft genome of S5 consisted of 7 048 502 bp, with a DNA G+C content of 44.9 % and that of Sa of 4 398 720 bp with a DNA G+C content of 67.9 %. The phylogenetic, phenotypic and physiological characteristics permitted the distinction of the two strains from their families, and we thus propose the names sp. nov. (type strain S5 = KACC 22689 = TBRC 16343) and sp. nov. (type strain Sa=KACC 22690 = TBRC 16344).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): draft genome , Dyella , paddy field , phylogenetic , polar lipid and Runella
© 2023 The Authors
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2023-06-26
2024-05-04
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Runella salmonicolor sp. nov. and Dyella lutea sp. nov., isolated from paddy field soil
Int J Syst Evol Microbiol 73, 005938 (2023); https://doi.org/10.1099/ijsem.0.005938
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