Nocardia sputorum sp. nov., an actinobacterium isolated from clinical specimens in Japan

Moriyuki Hamada; Narumi Enomoto; Tomonari Yamashita; Masahiro Shimojima; Daiki Tanno; Noboru Ohana; Masahiro Toyokawa; Hiroki Takahashi; Takashi Yaguchi

doi:10.1099/ijsem.0.005935

Volume 73, Issue 6

Research Article

Nocardia sputorum sp. nov., an actinobacterium isolated from clinical specimens in Japan

Moriyuki Hamada^1,2, Narumi Enomoto¹, Tomonari Yamashita³, Masahiro Shimojima⁴, Daiki Tanno^5,6, Noboru Ohana⁶, Masahiro Toyokawa^5,6, Hiroki Takahashi^2,7,8 and Takashi Yaguchi²
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Affiliations: ¹ Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan ² Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan ³ MicroSKY Lab, Inc., Center building Kanamachi 2F, 6-6-5 Higashikanamachi, Katsushika-ku, Tokyo 125-0041, Japan ⁴ SUGIYAMAGEN. CO., LTD., 2-34-9 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan ⁵ Department of Clinical Laboratory Sciences, School of Health Sciences, Fukushima Medical University, 10-6 Sakaemachi, Fukushima City, Fukushima 960-8516, Japan ⁶ Department of Laboratory Medicine, Fukushima Medical University, 1 Hikariga-oka, Fukushima City, Fukushima 960-1295, Japan ⁷ Molecular Chirality Research Center, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan ⁸ Plant Molecular Science Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan
*Correspondence: Moriyuki Hamada, [email protected]
Published: 16 June 2023 https://doi.org/10.1099/ijsem.0.005935

Abstract

Two novel actinobacteria, designated IFM 12276T and IFM 12275, were isolated from clinical specimens in Japan, and their taxonomic positions were investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strains IFM 12276 T and IFM 12275 have completely identical 16S rRNA gene sequences and were closely related to members of the genus Nocardia . The highest 16S rRNA gene sequence similarity was observed to Nocardia beijingensis (99.6 %) and Nocarida sputi (99.6 %), followed by Nocardia niwae (99.3 %) and Nocardia araoensis (99.3 %). The whole-cell hydrolysates of strains IFM 12276T and IFM 12275 contained meso-diaminopimelic acid, arabinose and galactose. The acyl type of muramic acid was N-glycolyl. The predominant isoprenoid quinone was MK-8(H4, ω-cycl.) and the principal polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. Strains IFM 12276T and IFM 12275 contained mycolic acids that co-migrated with those from the type strain of N. niwae . These chemotaxonomic features corresponded to those of the genus Nocardia . Meanwhile, the differences in some phenotypic characteristics, along with the results of average nucleotide identity and digital DNA–DNA hybridization analyses, indicated that strains IFM 12276 T and IFM 12275 should be distinguished from the recognized species of the genus Nocardia . Therefore, these strains represent a novel species of the genus Nocardia , for which the name Nocardia sputorum sp. nov. is proposed. The type strain is IFM 12276T (=NBRC 115477T=TBRC 17096T).

Received: 13/12/2022
Accepted: 03/05/2023
Published Online: 16/06/2023

Keyword(s): Nocardia , Nocardia sputorum , polyphasic taxonomy and sputum

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Nocardia sputorum sp. nov., an actinobacterium isolated from clinical specimens in Japan

Int J Syst Evol Microbiol 73, 005935 (2023); https://doi.org/10.1099/ijsem.0.005935

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Volume 73, Issue 6

Research Article

Nocardia sputorum sp. nov., an actinobacterium isolated from clinical specimens in Japan

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