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Volume 73, Issue 12

sp. nov., a hyperthermophilic archaeon isolated from a chimney in the Southwest Indian Ocean Open Access

Abstract

An anaerobic hyperthermophilic archaeon was isolated from a black smoker chimney with a snail attachment at a water depth of 2 739 m in the Southwest Indian Ocean. The sample was taken from the chimney exterior wall. The enrichment was conducted under a continuous culture with temperature fluctuation of 80–130 °C over 24 h for 42 days at 28 MPa. The isolation was performed at 90 °C at 0.1 MPa. Cells of the isolated strain 813A4 were irregular cocci. Strain 813A4 grew at 60–94 °C (optimal growth at 85 °C) at 0.1 MPa, and growth was detected at up to 99 °C at 28 MPa. At 85 °C, the strain was able to grow at pressures ranging from 0.1 to 110 MPa (optimal pressure, 0.1–40 MPa). At 85 °C, the cells of 813A4 grew at pH 5.5–9 (optimal, pH 7.0) and a NaCl concentration of 1.0–4.0 % (w/v; optimum concentration, 2.5 % NaCl). Strain 813A4 utilized yeast extract, tryptone and peptone as single carbon sources for growth. Elemental sulphur stimulated its growth. The G+C content of the complete genome was 53.48 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 813A4 belonged to the genus , with the highest sequence similarity to SHCK-94 (99.73 %). The average nucleotide identity between strains 813A4 and SHCK-94 was 82.56 %. All these data indicated that strain 813A4 should be classified as representing a novel species of the genus , for which sp. nov. is proposed. The type strain is 813A4 (=JCM 39367=MCCC M28628).

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  • Accepted:
  • Published Online:
Keyword(s): hydrothermal , hyperthermophilic , Southwest Indian Ocean and Thermococcus
Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 42106087)
    • Principle Award Recipient: WeishuZhao
  • Natural Science Foundation of China (Award 41921006)
    • Principle Award Recipient: XiangXiao
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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Thermococcus thermotolerans sp. nov., a hyperthermophilic archaeon isolated from a chimney in the Southwest Indian Ocean
Int J Syst Evol Microbiol 73, 005934 (2023); https://doi.org/10.1099/ijsem.0.005934
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/content/journal/ijsem/10.1099/ijsem.0.005934
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