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Abstract
Scotochromogenic slow-growing mycobacteria were isolated from the sputum or bronchoalveolar lavage fluid of 12 patients in Japan. From a comparison of the whole-genome sequences, the representative strain IWGMT90018-18076T and the unknown strains obtained from the patients were found to represent a novel species related to the Mycobacterium gordonae complex. The average nucleotide identity values of IWGMT90018-18076T with Mycobacterium vicinigordonae , Mycobacterium paragordonae and M. gordonae were 86.7, 82.5 and 82.2 %, respectively. The genome size of the representative strain IWGMT90018-18076T was approximately 6.3 Mbp, and the genomic DNA G+C content was 67.1 %. The major fatty acid methyl esters were C16 : 0 (37.71 %), C18 : 1ω9c (29.5 %) and C16 : 1ω7c (10.32 %). In this study, we performed phylogenetic analyses, physiological and biochemical characteristic tests, drug susceptibility tests and fatty acid profiling of the clinical isolates. On the basis of the results obtained, we propose that the unknown clinical isolates represent a novel species, ‘Mycobacterium kiyosense sp. nov,’ with the type strain being IWGMT90018-18076T (=JCM 34837T =KCTC 49725T).
- Received:
- Accepted:
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Funding
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Japan Society for the Promotion of Science
(Award JP20H02282)
- Principle Award Recipient: YoshihikoHoshino
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Japan Society for the Promotion of Science
(Award JP22K16382)
- Principle Award Recipient: HanakoFukano
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Japan Society for the Promotion of Science
(Award JP18K15966)
- Principle Award Recipient: HanakoFukano
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Japan Society for the Promotion of Science
(Award JP19KK0217)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22wm0325054)
- Principle Award Recipient: HanakoFukano
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Japan Agency for Medical Research and Development
(Award JP22wm0325054)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22wm0325003)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22wm0225022)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22wm0225004)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22wm0125007)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22gm1610003)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22fk0108608)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22fk0108129)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP22fk0108093)
- Principle Award Recipient: YoshihikoHoshino
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Japan Agency for Medical Research and Development
(Award JP21jm0510004)
- Principle Award Recipient: YoshihikoHoshino
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Japan Agency for Medical Research and Development
(Award JP20fk0108075)
- Principle Award Recipient: YoshihikoHoshino
-
Japan Agency for Medical Research and Development
(Award JP20fk0108064)
- Principle Award Recipient: YoshihikoHoshino