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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 5

sp. nov., isolated from marine sediment No Access

Abstract

A novel actinobacterium strain (M4I6) was isolated from marine sediment collected in Megas Gialos, Syros, Greece. On the basis of 16S rRNA gene sequence analysis, strain M4I6 was indicated as belonging to the genus , with high similarity to ‘’ LAM7112 (97.9 %), IFO 15555 (97.6 %), LIPI11-2-Ac042 (97.2 %) and LIPI11-2-Ac043 (97.2 %). Phylogenetic analysis of the 16S rRNA gene sequence of strain M4I6 showed that the strain formed a stable subclade with ‘’ LAM7112. The cell wall of the novel isolate contained -diaminopimelic acid and the whole-cell sugars were xylose, glucose and ribose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile comprised phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannosides and an unknown phospholipid. The major fatty acids (>5 %) were anteiso-C, iso-C, 10-methyl-C, C, iso-C and C. Genome sequencing showed a DNA G+C content of 70.9 mol%. However, the low average nucleotide identity value, digital DNA–DNA hybridization and average amino acid identity values demonstrated that strain M4I6 could be readily distinguished from its closest related species. Based on data from this polyphasic study, strain M4I6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M4I6 (=DSM 101017=CGMCC 4.7854).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA , Actinoplanes maris sp. nov. , marine sediment and polyphasic taxonomy
Funding
This study was supported by the:
  • China Agriculture Research System of MOF and MARA (Award CARS-21)
    • Principle Award Recipient: You-XingZhao
  • Financial Fund of the Ministry of Agriculture and Rural Affairs, P. R. of China (Award NFZX2021)
    • Principle Award Recipient: You-XingZhao
  • Natural Science Foundation of Hainan Province (Award 821RC643)
    • Principle Award Recipient: Qing-YiXie
© 2023 The Authors
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Actinoplanes maris sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 73, 005911 (2023); https://doi.org/10.1099/ijsem.0.005911
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