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Volume 73, Issue 5

sp. nov., a halotolerant actinobacterium isolated from Taklamakan desert soil No Access

Abstract

A Gram-stain-positive, aerobic, non-motile, non-spore-forming and rod-shaped actinobacterium, designated strain 10Sc9-8, was isolated from Taklamakan desert soil sampled in the Xinjiang Uygur Autonomous Region, China. Strain 10Sc9-8 grew at 8‒37 °C (optimum, 28‒30 °C), pH 6.0‒10.0 (optimum, pH 7.0–8.0) and in the presence of 0‒15 % (w/v) NaCl (optimum, 0–3 %). Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain 10Sc9-8 was affiliated with members of the genus and showed the highest 16S rRNA gene sequence similarity to Z443 (97.4 %). Phylogenomic analysis based on the whole genome sequences indicated that strain 10Sc9-8 should be assigned into the genus . The average nucleotide identity and digital DNA–DNA hybridization values calculated from the whole genome sequences indicated that strain 10Sc9-8 was clearly separated from other closely related species of the genus with values below the thresholds for species delineation. Chemotaxonomic analyses showed that the cell-wall peptidoglycan was in a variant of A4 type with an interpeptide bridge comprising -Lys–-Ala–Gly–-Asp. The predominant menaquinone was MK-8(H). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, several unidentified phospholipids, glycolipids and one unidentified lipid. The major fatty acids were anteiso-C, anteiso-C A and C. The genomic DNA G+C content was 72.7 mol%. On the basis of phenotypic, phylogenetic and phylogenomic data, strain 10Sc9-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 10Sc9-8 (=JCM 33946=CPCC 206219).

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  • Accepted:
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Keyword(s): 10Sc9-8T , Georgenia halotolerans , novel species , phylogenetic analysis and phylogenomic analysis
© 2023 The Authors
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2023-05-25
2024-05-01
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Georgenia halotolerans sp. nov., a halotolerant actinobacterium isolated from Taklamakan desert soil
Int J Syst Evol Microbiol 73, 005906 (2023); https://doi.org/10.1099/ijsem.0.005906
/content/journal/ijsem/10.1099/ijsem.0.005906
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