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Volume 73, Issue 6

sp. nov., a bacterium isolated from root-zone soil of a native legume, (L.) Fernald, in Quebec, Canada Open Access

Abstract

Four bacterial strains (S1Bt3, S1Bt7, S1Bt30 and S1Bt42) isolated from soil collected from the rhizosphere of a native legume, , were investigated using a polyphasic approach. Colonies were fluorescent, white-yellowish, circular and convex with regular margins on King’s B medium. Cells were Gram-reaction-negative, aerobic, non-spore-forming rods. Oxidase- and catalase-positive. The optimal growth temperature of the strains was 37 °C. Phylogenetic analysis of the 16S rRNA gene sequences placed the strains within the genus . Analysis of the 16S rRNA- concatenated sequences clustered the strains and well separated from CIP 104664 and CFM 97-514 with the type strains of the closest species. Phylogenomic analysis of 92 up-to-date bacterial core gene and matrix-assisted laser desorption/ionization-time-of-flight MS biotyper data confirmed the distinct clustering pattern of these four strains. Digital DNA–DNA hybridization (41.7 %–31.2 %) and average nucleotide identity (91.1 %–87.0 %) values relative to closest validly published species were below the species delineation thresholds of 70 and 96 %, respectively. Fatty acid composition results validated the taxonomic position of the novel strains in the genus . Phenotypic characteristics from carbon utilization tests differentiated the novel strains from closely related species. prediction of secondary metabolite biosynthesis gene clusters in the whole-genome sequences of the four strains revealed the presence of 11 clusters involved in the production of siderophore, redox-cofactor, betalactone, terpene, arylpolyene and nonribosomal peptides. Based on phenotypic and genotypic data, strains S1Bt3, S1Bt7, S1Bt30 and S1Bt42 represent a novel species for which the name sp. nov. is proposed. The type strain is S1Bt42 (=DOAB 746=LMG 32141=CECT 30251). The genomic DNA G+C content is 60.95 mol%.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Amphicarpaea bracteata , Canadian woodland soils , genome-based DNA–DNA hybridization , Pseudomonas , Pseudomonas quebecensis , whole genome and whole-genome average nucleotide identity
Funding
This study was supported by the:
  • Agriculture and Agri-Food Canada (Award J-002272, J-002295 and J-000409)
    • Principle Award Recipient: JamesT. Tambong
© 2023 Crown Copyright
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-06-16
2024-12-09
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Pseudomonas quebecensis sp. nov., a bacterium isolated from root-zone soil of a native legume, Amphicarpaea bracteata (L.) Fernald, in Quebec, Canada
Int J Syst Evol Microbiol 73, 005890 (2023); https://doi.org/10.1099/ijsem.0.005890
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