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Volume 73, Issue 5

sp. nov., a phycosphere bacterium isolated from No Access

Abstract

A facultative anaerobic, Gram-negative, non-motile, rod-shaped bacterial strain, designated N5, was obtained from the phycosphere microbiota of the marine planktonic dinoflagellate, . Strain N5 showed growth on marine agar at 25 °C, pH 7 and 1 % (w/v) NaCl and produced a yellow colour. According to a phylogenetic study based on 16S rRNA gene sequences, strain N5 has a lineage within the genus . The G+C content in the genome of strain N5 is 62.9 mol% with a total length of 4 324 088 bp. The NCBI Prokaryotic Genome Annotation Pipeline revealed that the N5 genome contained 4230 protein-coding genes and 48 RNA genes, including a 5S rRNA, 16S rRNA, 23S rRNA, 42 tRNA, and three ncRNAs. Genome-based calculations (genome-to-genome distance, average nucleotide identity and DNA G+C content) clearly indicated that the isolate represents a novel species within the genus . The predominant fatty acids were C cyclo 8 and feature 8 (comprising C 6 and/or C 7). The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The main respiratory quinone was Q-10. Based on its phenotypic, phylogenetic, genomic and chemotaxonomic features, strain N5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N5 (=KCTC 82362=NBRC 114899).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , phycosphere microbiota , polyphasic taxonomy and whole-genome sequence
Funding
This study was supported by the:
  • the KRIBB research initiative program (Award KGM5282331)
    • Principle Award Recipient: JiyoungLee
  • National Research Foundation of Korea (NRF) funded by the Ministry of Education (Award 2020R111A2072308)
    • Principle Award Recipient: JiyoungLee
© 2023 The Authors
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2023-05-04
2024-05-15
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Gymnodinialimonas phycosphaerae sp. nov., a phycosphere bacterium isolated from Karlodinium veneficum
Int J Syst Evol Microbiol 73, 005874 (2023); https://doi.org/10.1099/ijsem.0.005874
/content/journal/ijsem/10.1099/ijsem.0.005874
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