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Volume 73, Issue 5

gen. nov., sp. nov., a novel anaerobic bacterium isolated from human bronchoalveolar lavage fluid No Access

Abstract

Two related anaerobic strains, designated as SWB101512 and SWB19611, were isolated from the bronchoalveolar lavage fluid of two lung cancer patients. Cells were Gram-stain-positive, non-motile and non-spore-forming. Growth could be observed at 26–45 °C (optimum, 37 °C), pH 5.0–8.5 (optimum, pH 7.0) and with 0.5–2.0 % (v/w) NaCl (optimum, 1.0%). The 16S rRNA gene sequences of SWB101512 and SWB19611 showed the highest similarities to DSM 21843 (91.1 and 91.3 %, respectively). The phylogenetic tree based on the 16S rRNA gene sequences and the core genome sequences demonstrated that the two strains clustered together and formed a distinct lineage within the family . The DNA G+C contents of strains SWB101512 and SWB19611 were 62.0 and 61.9 mol%, respectively. The predominant cellular fatty acids of strains SWB101512 and SWB19611 were C DMA (27.8 and 28.8 %, respectively). The respiratory menaquinone in both strains was menaquinone 6 and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, three glycolipids and three unidentified lipids. Based on evidence from phenotypic, chemotaxonomic and genomic analyses, a new genus and species belonging to the family , named gen. nov., sp. nov. is proposed. The type strain is SWB101512 (=GDMCC 1.2991=JCM 35330).

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Keyword(s): Eggerthellaceae , human bronchoalveolar lavage fluid and polyphasic taxonomy
© 2023 The Authors
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2023-05-15
2024-05-13
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Curtanaerobium respiraculi gen. nov., sp. nov., a novel anaerobic bacterium isolated from human bronchoalveolar lavage fluid
Int J Syst Evol Microbiol 73, 005864 (2023); https://doi.org/10.1099/ijsem.0.005864
/content/journal/ijsem/10.1099/ijsem.0.005864
/content/journal/ijsem/10.1099/ijsem.0.005864
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