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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 5

sp. nov., an aerobic anoxygenic photoheterotrophic bacterium, isolated from surface seawater in the South China Sea No Access

Abstract

A Gram-stain-negative marine bacterium, designated as WX04, was isolated from the South China Sea. The genome of strain WX04 contained a complete photosynthetic gene cluster and is the first identified photoheterotroph of the genus with high photochemical efficiency (Fv/Fm=0.705±0.010), indicating its diverse metabolic and growth strategies, and unique evolution in the genus . The genome size of strain WX04 is 3.78 Mbp, and the G+C content is 58.8 %. Its isolate formed pink colonies and the cells were non-flagellated and rod-shaped. Growth was observed at 15–35 °C (optimum, 30 °C), at pH 5.0–11.0 (optimum, pH 7.0) and in the presence of 3–5 % (w/v) NaCl (optimum, 3 %). Both catalase activity and oxidase activity were found to be negative. The 16S rRNA gene sequence analyses revealed that this isolate represents a novel species within the genus , sharing 96.8 and 95.6% sequence identities with DSM 15283 and DSM 26895, respectively. The respiratory quinone was ubiquinone-10 (100 %). The primary cellular fatty acids (>5 %) were summed feature 8 (C 7 and/or C 6), C,C 7 11-methyl and C 3-OH. The dominant polar lipids of strain WX04 comprised phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. The combined polyphasic data shows that strain WX04 is a novel species within the genus , which is proposed as sp. nov., and the type strain is WX04 (=KCTC 62628=MCCC 1K02295).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): aerobic anoxygenic photosynthesis , Roseobacter and Shimia
Funding
This study was supported by the:
  • National Key Research Programs (Award 2018YFA0605800)
    • Principle Award Recipient: QiChen
  • Cross strait postdoctoral exchange and funding program of Fujian Province (Award K8318001)
    • Principle Award Recipient: Ta-HuiLin
  • Fundamental Research Funds for the Central Universities (Award 20720190095)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award 41776145, 41876150, 91751207 and 41861144018)
    • Principle Award Recipient: NotApplicable
© 2023 The Authors
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2023-05-12
2024-05-01
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Shimia ponticola sp. nov., an aerobic anoxygenic photoheterotrophic bacterium, isolated from surface seawater in the South China Sea
Int J Syst Evol Microbiol 73, 005859 (2023); https://doi.org/10.1099/ijsem.0.005859
/content/journal/ijsem/10.1099/ijsem.0.005859
/content/journal/ijsem/10.1099/ijsem.0.005859
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