1887

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Volume 73, Issue 4

sp. nov., a chitin-degrading actinobacterium, isolated from shrimp pond soil No Access

Abstract

A Gram-stain-positive actinobacterium, designated strain GXMU-J5, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China. The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus . The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores. Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5 was most related to DSM 41681 (similarity 97.74 %) and YIM 65594 (similarity 96.80 %). However, the values of digital DNA–DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5 and its closest relatives indicated that it represented a distinct species. Strain GXMU-J5 contained -diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose. The predominant menaquinones of strain GXMU-J5 were revealed as MK-9(H4), MK-9(H6) and MK-9(H8). The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine. The predominant cellular fatty acids were iso-C, anteiso-C, iso-C, iso-C and anteiso-C. The whole genome size of strain GXMU-J5 was 6.79 Mbp with a 71.39 mol% G+C content. Genomic analysis indicated that strain GXMU-J5 had the potential to degrade chitin. On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain GXMU-J5 (=MCCC 1K08064=JCM 35629).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chitinase , polyphasic taxonomy , shrimp pond soil and Streptomyces beihaiensis
Funding
This study was supported by the:
  • Science and Technology Major Project of Guangxi Province (CN) (Award AB221220020；AB21196019)
    • Principle Award Recipient: ZhangHongyan
  • Science and Technology Major Project of Guangxi Province (CN) (Award AA18242026)
    • Principle Award Recipient: ZhangHongyan
  • Natural Science Foundation of China (Award 31660022, 32060020)
    • Principle Award Recipient: ShenNaikun
  • Innovation Project of Guangxi Graduate Education (CN) (Award gxun-chxps202078)
    • Principle Award Recipient: NaikunShen
  • Guangxi Graduate Education Innovation Plan Project (CN) (Award YCSW2022256)
    • Principle Award Recipient: MingguoJiang
  • Research on experimental technology innovation and Laboratory management of Guangxi Minzu Univesity (Award 2019MDSY010, 2020MDSY006)
    • Principle Award Recipient: ShenNaikun
  • InnovationTeam Fund Project of Young Xiangsi Lake Scholars of Guangxi Minzu University (Award 2017-6)
    • Principle Award Recipient: JiangMingguo
  • Natural Science Foundation of Guangxi Province (CN) (Award 2019GXNSFAA185003)
    • Principle Award Recipient: HongyanZhang
© 2023 The Authors
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Streptomyces beihaiensis sp. nov., a chitin-degrading actinobacterium, isolated from shrimp pond soil
Int J Syst Evol Microbiol 73, 005857 (2023); https://doi.org/10.1099/ijsem.0.005857
/content/journal/ijsem/10.1099/ijsem.0.005857
/content/journal/ijsem/10.1099/ijsem.0.005857
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