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Abstract

A Gram-stain-negative, facultative anaerobic, motile, rod-shaped and orange bacterium, designated A06, was obtained off the coast of Weihai, PR China. Cells were 0.4–0.5×0.6–1.0 µm in size. Strain A06 grew at 20–40 °C (optimum, 33 °C), at pH 6.0–8.0 (optimum, pH 6.5–7.0) and in the presence of 0–8 % NaCl (w/v) (optimum, 2 %). Cells were oxidase and catalase positive. Menaquinone-7 was detected as the major respiratory quinone. The dominant cellular fatty acids were identified as C 2-OH, iso-C, anteiso-C and iso-C 6. The DNA G+C content of strain A06 was 46.1 mol%. The polar lipids were phosphatidylethanolamine, one aminolipid, one glycolipid and three unidentified lipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A06 is a member of the family and exhibited the highest sequence similarity to DSM 27148 (94.3 %). Based on its phylogenetic and phenotypic characteristics, strain A06 is considered to represent a novel genus in the family , for which the name gen. nov. is proposed. The type species is sp. nov., with type strain A06 (=KCTC 92029=MCCC 1H00491). The identification and acquisition of microbial species and genes in sediments will help broaden the understanding of microbial resources and lay a foundation for its application in biotechnology. Strain A06 uses an enrichment method, so the isolation of strain A06 is of great significance to the enrichment of marine microbial resources.

Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Da-Shuai Mu
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2023-05-03
2024-12-02
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