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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 5

gen. nov., sp. nov., isolated from coastal sediment No Access

Abstract

A Gram-stain-negative, facultative anaerobic, motile, rod-shaped and orange bacterium, designated A06, was obtained off the coast of Weihai, PR China. Cells were 0.4–0.5×0.6–1.0 µm in size. Strain A06 grew at 20–40 °C (optimum, 33 °C), at pH 6.0–8.0 (optimum, pH 6.5–7.0) and in the presence of 0–8 % NaCl (w/v) (optimum, 2 %). Cells were oxidase and catalase positive. Menaquinone-7 was detected as the major respiratory quinone. The dominant cellular fatty acids were identified as C 2-OH, iso-C, anteiso-C and iso-C 6. The DNA G+C content of strain A06 was 46.1 mol%. The polar lipids were phosphatidylethanolamine, one aminolipid, one glycolipid and three unidentified lipids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A06 is a member of the family and exhibited the highest sequence similarity to DSM 27148 (94.3 %). Based on its phylogenetic and phenotypic characteristics, strain A06 is considered to represent a novel genus in the family , for which the name gen. nov. is proposed. The type species is sp. nov., with type strain A06 (=KCTC 92029=MCCC 1H00491). The identification and acquisition of microbial species and genes in sediments will help broaden the understanding of microbial resources and lay a foundation for its application in biotechnology. Strain A06 uses an enrichment method, so the isolation of strain A06 is of great significance to the enrichment of marine microbial resources.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , Gaoshiqia sediminis and Prolixibacteraceae
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Da-Shuai Mu
© 2023 The Authors
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2023-05-03
2024-05-01
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Gaoshiqia sediminis gen. nov., sp. nov., isolated from coastal sediment
Int J Syst Evol Microbiol 73, 005855 (2023); https://doi.org/10.1099/ijsem.0.005855
/content/journal/ijsem/10.1099/ijsem.0.005855
/content/journal/ijsem/10.1099/ijsem.0.005855
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