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Volume 73, Issue 4

sp. nov., an endophytic actinobacterium with biosynthesis potential as an antibiotic producer No Access

Abstract

An endophytic actinobacterium, strain PIP175, was isolated from the root sample of a native apricot tree () growing on the Bedford Park campus of Flinders University, Adelaide, South Australia. This strain is a Gram stain-positive, aerobic actinobacterium with well-developed substrate mycelia. Aerial mycelia rarely produce spores and the spore chain is spiral. Strain PIP175 showed the highest 16S rRNA gene sequence similarity to DSM 41644 (99.4 %). Other closely related phylogenetic representatives include DSM 41902 (98.3 %), NBRC 13846 (97.6 %), subsp. NRRL B-1220 (97.5 %) and NBRC 14600 (97.4 %). The major cellular fatty acid of this strain was iso-C and the major menaquinone was MK-9(H). The whole-cell sugar contained galactose, glucose and mannose. Chemotaxonomic data confirmed that strain PIP175 belonged to the genus . Digital DNA–DNA hybridization, average nucleotide identity based on and OrthoANIu results between strain PIP175 and DSM 41644 were 60.0, 94.1 and 94.9 %, respectively. Genotypic and phenotypic data and genome analysis results allowed the differentiation of strain PIP175 from its closest species with validly published names. Strain PIP175 showed good activity against methicillin-resistant 03120385. Genome mining of strain PIP175 revealed biosynthetic genes encoding proteins relating to antibiotic production, plant growth promotion and biodegradation enzymes. The name proposed for the new species is sp. nov. The type strain is PIP175 (=DSM 103379=TBRC 6026).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): actinobacterium , endophyte , genome mining and Streptomyces phytophilus sp. nov.
Funding
This study was supported by the:
  • Mahasarakham University (Award 2022_Fast track)
    • Principle Award Recipient: OnumaKaewkla
© 2023 The Authors
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2024-05-14
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Streptomyces phytophilus sp. nov., an endophytic actinobacterium with biosynthesis potential as an antibiotic producer
Int J Syst Evol Microbiol 73, 005834 (2023); https://doi.org/10.1099/ijsem.0.005834
/content/journal/ijsem/10.1099/ijsem.0.005834
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