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Volume 73, Issue 3

sp. nov., isolated from mountain soil No Access

Abstract

A Gram-stain-positive, spore-forming and facultative aerobic bacterium, designated C11, was isolated from mountain soil collected in the Republic of Korea. The cells were motile rods with peritrichous flagella, and positive for catalase and oxidase activities. Strain C11 grew at 15–45 °C (optimum, 30–37 °C) and pH 6.0–8.0 (optimum, pH 6.0) and in the presence of 0–1 % (w/v) NaCl (optimum, 0.5 %). Strain C11 contained menaquinone-7 as the sole isoprenoid quinone and iso-C, iso-C and anteiso-C as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. The G+C content of the genomic DNA was 38.8 mol%. Strain C11 was most closely related to IDA1967 and CV53, with 98.0 and 97.7 %, 71.7 and 69.9 %, and 20.1 and 20.3 % 16S rRNA gene sequence similarity, average nucleotide identity, and digital DNA–DNA hybridization values, respectively. Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that strain C11 was included in a phyletic lineage with members of the genus but was distinct from members of the genus . Phenotypic, chemotaxonomic and molecular properties suggested that strain C11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C11 (=KACC 21661=JCM 33943).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillota , Neobacillus terrae , new taxa and soil
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award No. NIBR202203205)
    • Principle Award Recipient: CheOk Jeon
  • Chung-Ang University
    • Principle Award Recipient: CheOk Jeon
© 2023 The Authors
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2023-03-08
2024-04-27
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Neobacillus terrae sp. nov., isolated from mountain soil
Int J Syst Evol Microbiol 73, 005818 (2023); https://doi.org/10.1099/ijsem.0.005818
/content/journal/ijsem/10.1099/ijsem.0.005818
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