1887

Abstract

The novel, aerobic, Gram-stain-positive, rod-shaped bacterial strain, ZW T2_19, was isolated from an onion sample ( var. Rijnsburger). Analyses of the 16S rRNA gene sequence revealed that ZW T2_19 represented a member of the genus but may represent a novel species of this genus. Analyses of the whole draft genome sequences, i.e. digital DNA–DNA hybridisation (dDDH) and average nucleotide identity (ANI) of ZW T2_19 and all type strains of species of the genus confirmed that ZW T2_19 represents a novel species of the genus . The genome size of ZW T2_19 is 4.01 Mbp and the DNA G+C content is 71.8 mol%. Glucose, mannose, rhamnose and ribose were detected as whole-cell sugars of ZW T2_19. The major respiratory quinone of ZW T2_19 is menaquinone MK-10, at 78.9 %. The detected peptidoglycan type in ZW T2_19 is a variant of type B2γ with {Gly} [-diaminobutyric acid (-DAB)/-homoserine (-Hse)] -Glu--DAB. Polar lipids in ZW T2_19 consisted of one diphosphatidylglycerol, one phosphatidylglycerol, seven glycolipids, one phospholipid and one lipid. The fatty acid profile of ZW T2_19 predominantly consisted of anteiso-C (53 %), iso-C (21 %) and anteiso-C (18 %). In addition, API 20NE, API 50CH, API Coryne, API ZYM, antibiotic susceptibility, haemolysis and growth at different temperatures and with different supplements was investigated. On the basis of the results obtained using this polyphasic approach, including molecular, phenotypic and biochemical analyses, we propose the novel species with the type and only strain ZW T2_19 (= DSM 114294 = LMG 32700).

Funding
This study was supported by the:
  • Bundesanstalt für Landwirtschaft und Ernährung (Award 2819OE019)
    • Principle Award Recipient: NotApplicable
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-04-11
2024-12-07
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