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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov., an aerobic bacteriochlorophyll -containing freshwater bacterium isolated from river epilithic biofilm No Access

Abstract

A strictly aerobic bacteriochlorophyll -containing alphaproteobacterium, designated strain S08, was isolated from a biofilm sampled at Tama River in Japan. The non-motile and rod-shaped cells formed pink-beige pigmented colonies on agar plates containing organic compounds and showed absorption maxima at 798 and 866 nm in the near-infrared region, typical for the presence of bacteriochlorophyll . The new bacterial isolate is Gram-negative, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S08 was closely related to species in the genus . The closest phylogenetic relative of strain S08 was TH-G33 (98.2 % sequence similarity). The major cellular fatty acids were C, C 2-OH and summed feature 8 (C 7/C 6). The predominant respiratory quinone was ubiquinone-9. The major polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an aminolipid. The G+C content of the genomic DNA was 70.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain S08 and the related type strains were all far lower than the cut-off value for the delineation of species. The results of polyphasic comparisons showed that strain S08 was clearly distinguishable from other members of the genus . Therefore, we propose a new species in the genus , namely, sp. nov. The type strain is S08 (=DSM 111902=NBRC 112025).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): AAPB , Aerobic anoxygenic phototrophic bacteria , bacteriochlorophyll , BChl , epilithic biofilm and river
Funding
This study was supported by the:
  • Institute for Fermentation, Osaka
    • Principle Award Recipient: SatoshiHanada
© 2023 The Authors
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2023-03-31
2024-05-10
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Roseomonas fluvialis sp. nov., an aerobic bacteriochlorophyll a-containing freshwater bacterium isolated from river epilithic biofilm
Int J Syst Evol Microbiol 73, 005810 (2023); https://doi.org/10.1099/ijsem.0.005810
/content/journal/ijsem/10.1099/ijsem.0.005810
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