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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov. and sp. nov., isolated from marine sediment No Access

Abstract

Two Gram-stain-negative, strictly aerobic, chemoheterotrophic, short-rod-shaped and non-motile strains, forming yellow colonies and designated F47161 and F64183, were isolated from marine sediment of Xiaoshi Island, Wei Hai, PR China. Strain F47161 grew at 15–37 °C (optimally at 30 °C) and pH 6.0–9.0 (optimally at pH 7.5) and in the presence of 1–9 % (w/v) NaCl (optimally at 3 %). Strain F64183 grew at 10–37 °C (optimally at 30 °C) and pH 6.0–8.5 (optimally at pH 7.0) and in the presence of 1–8 % (w/v) NaCl (optimally at 3 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that F47161 and F64183 were related to members of the genus . The strains shared 97.4 % 16S rRNA gene sequence similarity to each other. F47161 and F64183 shared highest 16S rRNA gene sequence similarity to JCM 19789, and the values were 97.5 % and 98.4 %, respectively. The predominant cellular fatty acids of both F64183 and F47161 were iso-C and iso-C 3-OH, but the predominant fatty acids of F47161 also included anteiso-C. The sole respiratory quinone of F47161 and F64183 was menaquinone 6 (MK-6), consistent with that observed for all related strains. Between F47161 and F64183, the average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) values were 75.8 % and 20.5 %, respectively, and between the novel isolates (F47161 and F64183) and JCM 19789 they were 76.0 % and 94.2 % and 20.6 % and 57.1 %, respectively. The genomic DNA G+C contents of F47161 and F64183 was 37.3 % and 34.5 %, respectively. The polar lipid profiles of F47161 and F64183 contained phosphatidylethanolamine, two aminolipids, one glycolipid, one phosphoglycolipid and two unidentified polar lipids. Differential phenotypic and genotypic characteristics of the two strains indicated that the two strains should be classified as representing two novel species of the genus for which the names sp. nov. and sp. nov. are proposed. The type strains are F47161 (=MCCC 1H00509=KCTC 92017) and F64183 (=MCCC 1H00507=KCTC 92016), respectively.

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene , Aequorivita and phylogenetic analysis
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Da-ShuaiMu
© 2023 The Authors
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2023-03-24
2024-05-06
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Aequorivita vitellina sp. nov. and Aequorivita xiaoshiensis sp. nov., isolated from marine sediment
Int J Syst Evol Microbiol 73, 005801 (2023); https://doi.org/10.1099/ijsem.0.005801
/content/journal/ijsem/10.1099/ijsem.0.005801
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