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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 73, Issue 3

sp. nov., isolated from marine sponge No Access

Abstract

A novel bacterial strain, designated as PHS-Z21, was isolated from the marine sponge collected from PG Dave’s Rock, Philippines. Cells of PHS-Z21 are Gram-stain-negative, non-motile, pale-yellow-pigmented, short rods. PHS-Z21 is able to grow at 10–40 ℃ (optimum, 30 ℃), pH 5.5–9.0 (optimum, pH 8.5) and with 3–9 % (w/v) NaCl (optimum, 4 %). Its 16S rRNA gene sequence shows 98.6 % similarity to CGMCC 1.7715, 98.5 % similarity to 022-2-10 and 98.4 % similarity to SW-46, respectively. The phylogenetic tree based on 16S rRNA gene sequences reveals that PHS-Z21 is clustered with SW-46. The total genome of PHS-Z21 is approximately 2 932 896 bp in size with a DNA G+C content of 64.7 %. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among PHS-Z21 and other type strains are 70.0–77.3 % (ANIb), 83.3–86.8 % (ANIm) and 13.0–26.9 % (dDDH), respectively. The dDDH and ANI values are below the standard cutoff criteria for delineating bacterial species. Percentage of conserved proteins (POCP) values between the genome of strain PHS-Z21 and those of members of the genera , , and were 62.0–74.5 %, 55.8–63.2 %, 60.7–66.9 % and 63.9–66.8%, respectively, while the AAI values were 68.4–74.3 %, 63.8–65.9 %, 66.3–68.3 % and 64.7–66.9%, respectively. The major fatty acids of PHS-Z21 are composed of summed feature 8 (Cω7 and/or Cω6), Cω7 11-methyl, C and summed feature 3 (Cω7 and/or Cω6). The polar lipids of PHS-Z21 mainly consist of diphosphatidylglycerol, glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and glycophospholipid. The respiratory lipoquinone was identified as Q-10. On the basis of the phenotypic and phylogenetic data, strain PHS-Z21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PHS-Z21 (=MCCC 1K07849=KCTC 92590).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): marine sponge , polyphasic taxonomy and Qipengyuania spongiae sp. nov
Funding
This study was supported by the:
  • National Key Research and Development Program of China (Award 2018YFA0901901)
    • Principle Award Recipient: ZhiyongLi
© 2023 Shanghai Jiao Tong University
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2023-03-24
2024-05-08
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Qipengyuania spongiae sp. nov., isolated from marine sponge Cinachyrella kuekenthali
Int J Syst Evol Microbiol 73, 005800 (2023); https://doi.org/10.1099/ijsem.0.005800
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